Detail of "23491-52-3"

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Extracellular vesicles from non-pathogenic amoebae useful as vehicle for transferring a molecule of interest to a eukaryotic cell

Extracellular vesicles from non-pathogenic amoebae useful as vehicle for transferring a molecule of interest to a eukaryotic cell. Tatischeff, Irene; Alfsen, Annette; Lavialle, Francoise (Universite Pierre et Marie Curie Paris VI, Fr.). Eur. Pat. Appl.Some commonly used reagents like 23491-52-3 is used in this experiment. EP 1498144 A1 19 Jan 2005, 23 pp. DESIGNATED STATES: R: AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LI, LU, NL, SE, MC, PT, IE, SI, LT, LV, FI, RO, MK, CY, AL, TR, BG, CZ, EE, HU, SK. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: A61K047-48. APPLICATION: EP 2003-291752 15 Jul 2003. DOCUMENT TYPE: Patent CA Section: 63 (Pharmaceuticals) Section cross-reference(s): 15 The invention relates to membrane vesicles from a non-pathogenic amoeba, in particular from the amoeba Dictyostelium discoideum, contg. a mol. of interest, to a method of prepg. such vesicles and to the uses of said vesicles as a vehicle for transferring the mol. of interest to a eukaryotic cell. The technique may be adapted to drug delivery, immunization, etc. .

Membrane-permeant, DNA-binding agents alter intracellular trafficking and increase the transfection efficiency of complexed plasmid DNA

Membrane-permeant, DNA-binding agents alter intracellular trafficking and increase the transfection efficiency of complexed plasmid DNA. Fong, Sylvia; Liu, Yong; Heath, Timothy; Fong, Paul; Liggitt, Denny; Debs, Robert J. (California Pacific Medical Center Research Institute, San Francisco, CA 94115, USA). Molecular Therapy, 10(4), 706-718 (English) 2004 Elsevier. CODEN: MTOHCK. 23491-52-3 and 144189-73-1 are cas registry numbers. These chemicals are also mentioned in this article. ISSN: 1525-0016. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Nuclear delivery of extracellular DNA by nonviral vectors is inhibited by a series of cell membrane and compartmental barriers. Certain cationic amphiphiles that partition through cellular membranes to bind genomic DNA can enhance nuclear delivery of plasmid DNA. Specifically, delivering plasmid DNA complexed to the DNA-binding dye Hoechst 33258 produces cellular transfection levels similar to those achieved by cationic liposome:DNA complexes (CLDC), with less toxicity. Incorporating Hoechst into CLDC or polyethyleneimine:DNA complexes significantly increased reporter gene expression, as well as the percentage of cells transfected. Hoechst:CLDC significantly improved transfection of nondividing cells and efficiently transfected cells in the presence of anionic mols. that block cellular uptake of and transfection by CLDC alone. Hoechst:CLDC also increased gene expression in mouse tissues following i.v. delivery. Delivery of fluorescently labeled plasmid DNA via Hoechst altered its intracellular trafficking by both minimizing lysosomal sequestration and accelerating delivery into the nucleus. Agents such as Hoechst constitute a novel class of nonviral carriers that can confer their membrane-permeant properties on complexed DNA, thus redirecting its intracellular trafficking. In addn., binding of Hoechst 33258 to specific chromosomal DNA target sequences and its ability to modulate transcription may further enhance the expression of delivered genes. .