Detail of "2398-34-7"

Reference

Limitations in the use of 3-hydroxy fatty acid analysis to determine endotoxin in mammalian samples

Limitations in the use of 3-hydroxy fatty acid analysis to determine endotoxin in mammalian samples. Szponar, Bogumila; Norin, Elisabeth; Midtvedt, Tore; Larsson, Lennart ( Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw 53-114, Pol.). Journal of Microbiological Methods, 50(3), 283-289 (English) 2002 Elsevier Science B.V. CODEN: JMIMDQ. ISSN: 0167-7012. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) 3-Hydroxy fatty acids (3-OH FAs) of 10-18-carbon chain lengths are constituents of the lipopolysaccharide of Gram-neg. bacteria. These acids are used as chem. markers for detg. endotoxin in environmental samples. The present communication addresses the question whether this type of anal. also would be applicable to mammalian samples. Low levels (6.1±1.6-94.0±23.2 pmol/mL) of the studied 3-OH FAs were detected in blood from both conventional and germ-free rats. The levels were considerably higher (0.0-1.06±0.17 nmol/mg) in livers. The amts. of the 3-OH FAs did not differ between the two groups of rats. All analyses were made by gas chromatog.-tandem mass spectrometry (GC-MSMS) for unequivocal identification. The results illustrate a limitation in using 3-OH FA anal. 17773-30-7 and 2398-34-7 are just another two chemicals used in this study. to det. endotoxin in mammalian samples since these acids may represent not only endotoxin but also products from mammalian mitochondrial fatty acid b-oxidn. .

Structural studies on the phosphate-free lipid A of Rhodomicrobium vannielii ATCC 17100

Structural studies on the phosphate-free lipid A of Rhodomicrobium vannielii ATCC 17100. Holst, Otto; Borowiak, Dietmar; Weckesser, Juergen; Mayer, Hubert (Max-Planck-Inst. Immunbiol., Freiburg, Fed. Rep. Ger.). Eur. J. Biochem., 137(1-2), 325-32 (English) 1983. CODEN: EJBCAI. ISSN: 0014-2956. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) The structure of the free lipid A from the lipopolysaccharide of R. vannielii ATCC 17100 was elucidated. It consists of a central b-1',6-linked glucosamine disaccharide which is not substituted by phosphate. About 30% of the disaccharide mols. are substituted with mannopyranose in b-1,4'-linkage to the nonreducing glucosamine. The reducing glucosamine can be directly reduced with NaBH4, indicating either that this glucosamine is not substituted at C1, or that its substituent has been removed during the prepn. of free lipid A or is removed during redn. with NaBH4. The following formula shows the backbone structure of the free lipid A from R. vannielii ATCC 17100: b-Manp-(1?4)-b-GlcpN-(1?6)-GlcpN. 3-(R)-Hydroxyhexadecanoic acid is linked to the amino group of the reducing glucosamine. The residue at the amino group of the nonreducing glucosamine was not identified. The OH groups of the central disaccharide are acylated with 3-(tetradecanoyloxy)tetradecanoic acid, 3-hydroxytetradecanoic acid, D14-docosenoic acid, and Ac groups. The OH groups of the mannose are not substituted.Except for chemicals metioned above, 2398-34-7 and 544-63-8 are also used. .