Detail of "24198-95-6"

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Substrate Specificity for the Epoxidation of Terpenoids and Active Site Topology of House Fly Cytochrome P450 6A1

Substrate Specificity for the Epoxidation of Terpenoids and Active Site Topology of House Fly Cytochrome P450 6A1. Andersen, John F.; Walding, Jennifer K.; Evans, Philip H.; Bowers, William S.; Feyereisen, Rene (Department of Entomology, University of Arizona, Tucson, AZ 85721, USA). Chemical Research in Toxicology, 10(2), 156-164 (English) 1997 American Chemical Society. CODEN: CRTOEC. ISSN: 0893-228X. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) Heterologous expression in Escherichia coli, purifn., and reconstitution of house fly P 450 6A1 and NADPH-cytochrome P 450 reductase were used to study the metab.There are some reagents like 24198-95-6 is used in this study. of terpenoids. In addn. to the epoxidn. of cyclodiene insecticides demonstrated previously [Andersen et al. (1994) Biochem. 33, 2171-2177], this cytochrome P 450 was shown to epoxidize a variety of terpenoids such as farnesyl, geranyl, and neryl Me esters, juvenile hormones I and III, and farnesal but not farnesol or farnesoic acid. P 450 6A1 reconstituted with NADPH-cytochrome P 450 reductase and phosphatidylcholine did not metabolize a-pinene, limonene, or the insect growth regulators hydroprene and methoprene. The four geometric isomers of Me farnesoate were metabolized predominantly to the 10,11-epoxides, but also to the 6,7-epoxides and to the diepoxides. The 10,11-epoxide of Me (2E,6E)-farnesoate was produced in a 3:1 ratio of the (10S) and (10R) enantiomers. Monoepoxides of Me farnesoate were metabolized efficiently to the diepoxides. Me farnesoate epoxidn. was strongly inhibited by a bulky substituted imidazole. The active site topol. of P 450 6A1 was studied by the reaction of the enzyme with phenyldiazene to form a phenyl-iron complex. Ferricyanide-induced in situ migration of the Ph group showed formation of the N-phenylprotoporphyrinporphyrin IX adducts in a : ratio of the NB:NA:NC:ND isomers. These expts. suggest that metab. of xenobiotics by this P 450, constitutively overexpressed in insecticide-resistant strains of the house fly, is not severely limited by stereochem. constrained access to the active site. .

Comparison of C18 (JH-I) and C16 (JH-III) juvenile hormone activities on the gonadotropic function of Locusta migratoria

Comparison of C18 (JH-I) and C16 (JH-III) juvenile hormone activities on the gonadotropic function of Locusta migratoria. Roussel, Jean P. (Lab. Biol. Gen., Univ. Louis-Pasteur, Strasbourg, Fr.). C. R. Hebd. Seances Acad. Sci., Ser. D, 283(8), 947-9 (French) 1976. CODEN: CHDDAT. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) The chronology of oocyte development in L. migratoria females deprived of their corpora allata was studied after injection of juvenile hormones. Juvenile hormone (Manduca sexta III) [61425-41-0] had a more rapid but much more limited action than juvenile hormone (Hyalophora cecropia I) [13804-51-8]. After hormone III, oocytes first showed rapid growth, then signs of degeneration appeared, and mature oocytes were rarely obtained. 24198-95-6 and 13804-51-8 are just another two chemicals used in this study. After hormone I, synchronous oocytes appeared in all cases after day 4; mature eggs formed; and the incidence of degeneration was the same as in controls. .