Reference

Active oxygen and anti-inflammatory drugs: measurement by luminol-dependent chemiluminescence

Active oxygen and anti-inflammatory drugs: measurement by luminol-dependent chemiluminescence. Mibu, Hiroyuki; Hasegawa, Junichi; Niwa, Masayuki; Nozaki, Masakatsu; Tsurumi, Kaito; Fujimura, Hajime (Sch. Med., Gifu Univ., Gifu 500, Japan). Nippon Yakurigaku Zasshi, 83(4), 355-62 (Japanese) 1984. CODEN: NYKZAU. ISSN: 0015-5691. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Active O produced from stimulated phagocytic cells emits luminol-dependent chemiluminescence (CL) on reaction with luminol. Active O can be assayed using CL, and the results are comparable to those obtained by the lactate dehydrogenase-NADH (LDH-NADH) method. The effects of nonsteroidal anti-inflammatory drugs on the generation of active O were examd. 69-72-7 and 15307-86-5 are also in the experiment. using both the CL and LDH-NADH methods. Peritoneal and pleural exudate cells (PEEG and PLEG) of rats emitted strong CL on incubation with zymosan, but whole blood showed weak CL. The effects of superoxide dismutase, catalase, NaN3, and L-ascorbic acid on the generation of active O emitted from phagocytic cells of rats were different as measured by CL and LDH-NADH methods. Salicylic acid [69-72-7], aspirin [50-78-2], phenylbutazone [50-33-9], flufenamic acid [530-78-9], indomethacin [53-86-1], diclofenac [15307-86-5], ketoprofen [22071-15-4], benoxaprofen [51234-28-7], suprofen [40828-46-4], clidanac [34148-01-1], tinoridine [24237-54-5], tiaramide [32527-55-2], mepirizole [18694-40-1], and perisoxal [2055-44-9] but not BW-755C [66000-40-6] had only slight inhibitory effects on the generation of active O. .

Effects of tinoridine on lipid peroxidation and renin release in the rat renin granule fraction

Effects of tinoridine on lipid peroxidation and renin release in the rat renin granule fraction. Matsumura, Yasuo; Miyawaki, Nobuaki; Ohno, Yukihiro; Sasaki, Yasuto; Shimizu, Toshikatsu; Morimoto, Shiro (Dep. Pharmacol., Osaka Coll. Pharm., Matsubara 580, Japan). J. Pharmacobio-Dyn., 8(7), 532-8 (English) 1985. CODEN: JOPHDQ. ISSN: 0386-846X. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Incubation of the renin granule fraction of rat kidney cortex at 37° with tinoridine (I) [24237-54-5] resulted in an increase in lipid peroxide formation, accompanied by increased release of renin [9015-94-5] from the granules. When the renin granule fraction was incubated with 50 mM tinoridine at 37 °, lipid peroxide formation in this fraction was completely inhibited. Simultaneously, the rate of renin release from the granules was significantly suppressed. Tinoridine, at concns. from 5 mM up to 100 mM, produced a concn.-dependent inhibition on the simultaneous increases in lipid peroxide formation and renin release induced by 50 mm ascorbic acid in the renin granule fraction. On the other hand, indomethacin, hydrocortisone, and prednisolone, which had no ability to inhibit the lipid peroxidn. in the renin granule fraction, did not influence the release of renin from the granules. Apparently, tinoridine suppresses renin release by inhibiting the oxidative disintegration of membranes of renin granules.