Reference

Guanine ribonucleotide depletion in mammalian cells

Guanine ribonucleotide depletion in mammalian cells. A target of purine antimetabolites. Nguyen, Binh T.; Cohen, Marvin B.; Sadee, Wolfgang (Sch. Pharm., Univ. California, San Francisco, CA 94143, USA).In this article, certain chemicals are used. Some of their cas registry numbers are 86-01-1 and 2564-35-4 Cancer Chemother. Pharmacol., 11(2), 117-19 (English) 1983. CODEN: CCPHDZ. ISSN: 0344-5704. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 14 Mycophenolic acid [24280-93-1]-induced guanine [73-40-5] starvation caused a drastic DNA synthesis inhibition in the human lymphoblastic T leukemia (CEM) and the mouse B leukemia (L1210) cell lines, which was assocd. with GTP [86-01-1] depletion rather than dGTP [2564-35-4] depletion. Apparently, GTP depletion represents a common target of purine antimetabolites in mammalian cells. .

Consequences of inhibition of guanine nucleotide synthesis by mycophenolic acid and virazole

Consequences of inhibition of guanine nucleotide synthesis by mycophenolic acid and virazole. Lowe, Jeffrey K.; Brox, Larry; Henderson, J. Frank (Dep. Biochem., Univ. Alberta, Edmonton, Alberta, Can.). Cancer Res., 37(3), 736-43 (English) 1977.There are some reagents with their cas registry numbers 65-47-4 and 9028-93-7 are used in this study. CODEN: CNREA8. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Mycophenolic acid (I) [24280-93-1] and virazole (II) [36791-04-5] were inhibitors of inosinate dehydrogenase [9028-93-7] and produced growth inhibition and loss of viability in cultured murine lymphoma L5178Y cells. Treatment with 1 .mu.M I produced the following changes in concns. of acid-sol. nucleotides: (a) guanosine triphosphate [86-01-1] decreased to less than 10% of control within 2 h; (b)uridine triphosphate [63-39-8] and cytidine triphosphate [65-47-4] concns. increased markedly; (c) ATP [56-65-5] did not change; (d) deoxyguanosine triphosphate [2564-35-4] decreased; and (e) thymidine triphosphate [365-08-2] increased. DNA synthesis was inhibited by 90% within 2 h, whereas the incorporation of adenosine into RNA and of leucine into protein were much leess affected. II (100 .mu.M) produced similar effects. These biochem. effects of I, as well as its effects on cell growth, could be prevented by addn. of guanylate to the medium. I treatment also appeared to cause breakdown of high-mol.-wt. DNA. .