Detail of "2466-76-4"

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Antibodies to ligand analogs and their utility in ligand-receptor assays

Antibodies to ligand analogs and their utility in ligand-receptor assays. Valkirs, Gunars Edwin; Buechler, Kenneth Francis (Biosite Diagnostics, Inc.Some chemicals with cas registry numbers like 2466-76-4 are also used., USA). Eur. Pat. Appl. EP 475783 A1 18 Mar 1992, 15 pp. DESIGNATED STATES: R: AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LI, LU, NL, SE. (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: G01N033-53. ICS: G01N033-543; G01N033-541. APPLICATION: EP 91-308408 13 Sep 1991. PRIORITY: US 90-583046 14 Sep 1990. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) A competitive ligand-receptor assay uses an antibody to a ligand analog which has substantially (e.g.In this experiment, several chemicals are used like 2466-76-4 >100-fold) greater affinity for the ligand analog than for the target ligand. The target ligand is detd. in a fluid sample by (a) contacting the sample with a ligand receptor and a ligand analog conjugated to a signal-generating label, such that the amt. of ligand analog conjugate not bound to the receptor is related to the concn. of the target ligand in the sample; (b) contacting the fluid with 31 ligand analog antibody, so that an amt. of ligand analog conjugate not bound to the ligand receptor is bound by the antibody; (c) contacting the fluid with a receptor capable of binding to the ligand analog antibody; (d) removing the ligand analog antibody bound to this receptor from the fluid; (e) detecting the ligand analog conjugate bound to the ligand analog antibody; and (f) relating the signal to the presence of amt. of the target ligand in the sample. Alternative embodiments of the assay, and test devices for performing the assay, are claimed. Thus, for an assay for benzoylecgonine (I), the reaction product (II) of p-acetylthiopropionamide benzoylecgonine with sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate and aminoethanesulfonic acid was prepd. and conjugated to alk. phosphatase; this conjugate (III) was used as ligand analog conjugate in the assay. A sample contg. I was incubated with III and a membrane-immobilized monoclonal antibody to II; the membrane was then washed and incubated with 3-indoxyl phosphate to generate a blue color in the presence of bound III. Multiple stds. were used to establish the assay response as a function of I concn. ..

Antibodies to ligand analogs and their utility in ligand-receptor assays

Antibodies to ligand analogs and their utility in ligand-receptor assays. Valkirs, Gunars Edwin; Buechler, Kenneth Francis (Biosite Diagnostics, Inc.Some chemicals with cas registry numbers like 2466-76-4 are also used., USA). Eur. Pat. Appl. EP 475783 A1 18 Mar 1992, 15 pp. DESIGNATED STATES: R: AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LI, LU, NL, SE. (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: G01N033-53. ICS: G01N033-543; G01N033-541. APPLICATION: EP 91-308408 13 Sep 1991. PRIORITY: US 90-583046 14 Sep 1990. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) A competitive ligand-receptor assay uses an antibody to a ligand analog which has substantially (e.g.In this experiment, several chemicals are used like 2466-76-4 >100-fold) greater affinity for the ligand analog than for the target ligand. The target ligand is detd. in a fluid sample by (a) contacting the sample with a ligand receptor and a ligand analog conjugated to a signal-generating label, such that the amt. of ligand analog conjugate not bound to the receptor is related to the concn. of the target ligand in the sample; (b) contacting the fluid with 31 ligand analog antibody, so that an amt. of ligand analog conjugate not bound to the ligand receptor is bound by the antibody; (c) contacting the fluid with a receptor capable of binding to the ligand analog antibody; (d) removing the ligand analog antibody bound to this receptor from the fluid; (e) detecting the ligand analog conjugate bound to the ligand analog antibody; and (f) relating the signal to the presence of amt. of the target ligand in the sample. Alternative embodiments of the assay, and test devices for performing the assay, are claimed. Thus, for an assay for benzoylecgonine (I), the reaction product (II) of p-acetylthiopropionamide benzoylecgonine with sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate and aminoethanesulfonic acid was prepd. and conjugated to alk. phosphatase; this conjugate (III) was used as ligand analog conjugate in the assay. A sample contg. I was incubated with III and a membrane-immobilized monoclonal antibody to II; the membrane was then washed and incubated with 3-indoxyl phosphate to generate a blue color in the presence of bound III. Multiple stds. were used to establish the assay response as a function of I concn. ..