Detail of "25535-16-4"

Reference

Transmitter specific mapping of neuronal pathways by immunohistochemistry combined with fluorescent dyes

Transmitter specific mapping of neuronal pathways by immunohistochemistry combined with fluorescent dyes. Skirboll, L.; Hoekfelt, T. (Dep. Histol., Karolinska Inst., Stockholm S-10401, Swed.). IBRO Handb. Ser., 3(Immunohistochemistry), 465-76 (English) 1983. CODEN: IHSED6. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 7 Fluorescent tracers, injection, survival times, tissue prepn., immunohistochem., and visualization are described for neurotransmitters or their synthesizing enzymes detection for nervous system pathway mapping.In this experiment, several chemicals are used like 9036-22-0 and 71431-30-6 The recommended procedure involved dye (Fast Blue [74749-42-1], True blue [71431-30-6], Propidium iodide [25535-16-4], or Primuline [8064-60-6]) injection; perfusion of the animal through the ascending aorta with 10% formalin; dissection, fixation, and sectioning of the brain; and immunofluorescence staining with the appropriate antiserum and FITC-conjugated antibodies. Examples are given of tyrosine hydroxylase [9036-22-0] and 5-HT [50-67-9] detection. .

Petite and sectored induction in Saccharomyces cerevisiae by propidium iodide: synergistic effect of sodium dodecyl sulfate

Petite and sectored induction in Saccharomyces cerevisiae by propidium iodide: synergistic effect of sodium dodecyl sulfate. Iwamoto, Yoshihisa; Yielding, K. Lemone (Dep. Anat., Univ. South Alabama, Mobile, AL 36688, USA). Mutat. Res., 126(2), 145-51 (English) 1984. CODEN: MUREAV. ISSN: 0027-5107. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Na dodecyl sulfate (SDS) [151-21-3] was examd. for its effect on petite and sectored colony induction in S. cerevisiae by propidium iodide (PI)(I) [25535-16-4] and ethidium bromide (EB)(II) [1239-45-8]. Four-hour cultivation with 100 mM PI and 100 mg/mL SDS resulted in virtually all plated cells growing as sectored colonies with no decrease in viability. Sectored colonies are mixed colonies comprised of respiratory-deficient and -competent cells believed to be derived from an unstable respiratory-deficient cell. Further cultivation with PI and SDS prior to plating led to induction of complete petite colonies with a rapid decrease in viable cells. PI alone at this concn. exhibited weak induction of sectored colonies and petite colonies, but SDS alone caused induction of neither. PI (50 mM) had almost the same activity as 100 mM except for a delay in the induction of sectored colonies in the initial stage, and a decreased rate of petite colony induction. The effects of 20 mM PI and SDS were much lower than that by 50 mM and no inhibition of growth was obsd. PI (10 mM) was quite inactive even in the presence of SDS. Under resting conditions, 10~100 mM PI and 100 mg/mL SDS induced ~60% sectored colonies at 12-h incubation and >60% petite colonies at 24 h. After 6-h incubation, decrease in survival was also obsd.