Detail of "26063-00-3"

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Insertion and deletion mutations within the nif region of Rhizobium japonicum

Insertion and deletion mutations within the nif region of Rhizobium japonicum. Hahn, Matthias; Meyer, Linda; Studer, Daniel; Regensburger, Brigitte; Hennecke, Hauke (Eidg. Tech. Hochsch., ETH-Zent., Zurich CH-8092, Switz.). Plant Mol. Biol., 3(3), 159-68 (English) 1984. CODEN: PMBIDB. ISSN: 0167-4412. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Insertion and deletion mutants were used to characterize a genomic region of R. japonicum where the nitrogenase [9013-04-1] structural genes are located on 2 sep. operons nifDK and nifH. In addn. to previously described nifD::Tn5 and nifK::Tn5 mutations, the authors have now generated, by localized mutagenesis, further Tn5 insertion mutations in the vicinity of nifDK as well as within and adjacent to nifH. The nifD::Tn5, nifK::Tn5, and nifH::Tn5 mutant strains were of the Nod+ Fix- phenotype, whereas all other mutants were symbiotically fully effective (Nod+ Fix+). The nifH::Tn5 mutation was helpful in the identification of the nifH gene product (the dinitrogenase reductase), by 2-dimensional gel electrophoresis: due to its polar effect, this insertion specifically abolished the synthesis of that protein under microaerobic culture conditions. The ultrastructure of soybean root nodules infected with either the nif+ wild-type or with the nif- (but otherwise isogenic) mutant strains was analyzed by electron microscopy. All contained fully developed bacteroids, but the N non-fixing mutants showed massive accumulation of poly-b-hydroxybutyric acid [26063-00-3]. Of the Tn5-contg. strains, kanamycin [59-01-8]-sensitive derivs. were obtained which contained deletions. Several classes of deletion mutants were found which, as judged by their phys. DNA structure and their phenotypes, allowed the following most important conclusions: (1) deletions lacking both the nifDK and nifH regions indicate linkage between the 2 operons whereby 315 kb of DNA sep. them; (2) one deletion ending upstream from nifH and lacking only nifDK, indicates that the nifDK operon is located on the 5'-flanking side of the nifH operon; (3) all deletion mutants are Nod+, indicating that there are no essential nodulation genes located between and adjacent to nifDK and nifH.

b-Hydroxybutyrate polymers by microbial cultivation

b-Hydroxybutyrate polymers by microbial cultivation. Richardson, Kenneth Raymond (Imperial Chemical Industries PLC, UK). Eur. Pat. Appl. EP 114086 A2 25 Jul 1984, 17 pp. DESIGNATED STATES: R: BE, CH, DE, FR, GB, IT, LI, NL. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: IC: C12P007-62; C08G063-06. APPLICATION: EP 84-300003 3 Jan 1984. PRIORITY: GB 83-1344 18 Jan 1983. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Poly-b-hydroxybutyrate (I) [26063-00-3] in produced by Alcaligenes eutrophus cultured on the hydrolyzate of its own cellular material ofter I had been extd. Thus, A. eutrophus NCHB 11599 was cultured on a conventional glucose-contg. medium to produce cells contg. ~50% I by wt. The cells were dried and extd. with CHCl4 to remove I. The cell residue was refluxed for 24 h in 6N HCl. The hydrolyzate was neutralized, filtered, dild., and used as the medium for A. eutrophus. After culturing aerobically at 34° for 48 h, 84% of the sol. C in the medium was utilized to produce cells contg. ~40% I.