Detail of "26336-38-9"

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Recovery and purification of a substance developed but not excreted by cells, by encapsulation and lysis of the cell membrane

Recovery and purification of a substance developed but not excreted by cells, by encapsulation and lysis of the cell membrane. Jarvis, Allan Perley, Jr. (Damon Biotech, Inc., USA). Fr. Demande FR 2544330 A1 19 Oct 1984, 27 pp. (French). (France). CODEN: FRXXBL. CLASS: IC: C12N011-04; C12N001-00; C12N005-00; C12P001-00. APPLICATION: FR 84-5948 13 Apr 1984. PRIORITY: US 83-485472 15 Apr 1983. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) The sepn. of metabolites formed in cell cultures is simplified by culturing the cells in membrane-covered alginate capsules, lysing the cells, and allowing the metabolites to diffuse into the extracapsular medium, sepd. by mol. size. Thus, penicillinase [9001-74-5] (mol. wt. 30,000) was produced and sepd. from Escherichia coli which also produces a lipopolysaccharide pyrogen of mol. wt. >50,000. The cells were immobilized in Ca alginate beads, which were then covered by semipermeable membranes of poly-L-lysine [25104-18-1] and then polyvinylamine [26336-38-9]. The gel was liquefied by suspending the capsules in a Na citrate soln. The capsules were then incubated at 37° for 24 h in Luria medium contg. Na citrate to prevent the gel from solidifying. The cells were lysed by suspending the capsules in 4M guanidine HCl [50-01-1] and the intracellular metabolites diffused into the intracapsular and extracapsular media. The rate and extent of cell growth and penicillinase prodn. were identical with that of free cells. After 24 h from cell lysis, 75% of the penicillinase had diffused into the extracapsular medium, while 90-95% of the pyrogen had been retained.

Synthesis of poly(vinylamine) derivatives and their complex formations with cupric ion

Synthesis of poly(vinylamine) derivatives and their complex formations with cupric ion. Kim, Woo Sik; Kang, Inn Kyu; Suh, Ho Jung; Shim, Sang Chul (Dep. Ind. Chem., Kyungpook Natl. Univ., Taegu, S. Korea). Pollimo, 9(1), 24-9 (Korean) 1985. CODEN: POLLDG. ISSN: 0379-153X. DOCUMENT TYPE: Journal CA Section: 35 (Chemistry of Synthetic High Polymers) Partially N-methylated poly(vinylamine) was synthesized by the reaction of poly(vinylamine) (I) [26336-38-9] with HCHO [50-00-0] in the presence of KHFe(CO)4. The degree of methylation, detd. by NMR, for derivs. prepd. in the ratios of 0.5 and 1:1 (molar ratio) HCHO-I were ~45 and 62%, resp. The chelations of these derivs. with Cu2+ and I-Cu2+ complex were investigated as a function of pH. The chelations of the deriv. systems occurred at higher pH than did the chelation of the amine system. Also, the visible spectra absorbances of the deriv. complexes were less intense and the max. absorption wavelengths of the deriv. complexes were shifted to longer wavelength. These results can be attributed to formation of an unstable complex due to the steric hindrance of Me group.