Detail of "27255-72-7"

Reference

Cellulose triacetate as an embedding agent for penicillin acylase producing cells of Escherichia coli

Cellulose triacetate as an embedding agent for penicillin acylase producing cells of Escherichia coli. Kou, Xiufen; Wang, Zhengxiang (Inst. Microbiol., Acad. Sin., Beijing, Peop. Rep. China). Weishengwuxue Tongbao, 11(4), 151-3 (Chinese) 1984. CODEN: WSWPDI. ISSN: 0253-2654. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) Cellulose triacetate [9012-09-3] (1 g) was dissolved in 20 mL CHCl3, mixed with 16 g wet cells of penicillin acylase [9014-06-6]-producing E. coli AS 1.76, then squeezed through a small bore into toluene. Spherical granules (~2 mm diam.) obtained were treated with CHO(CH2)3CHO for 1 h, washed, and packed in a column. Phenylacetyl-7-aminodeacetoxycephalosporanic acid (I) [27255-72-7] (5% soln., pH 7.7) was recycled through the column at 37°. After 3 h, >95% I was transformed to 7-aminodeacetoxycephalosporanic acid (II) [22252-43-3]. After 44 days operation for 40 batches of I, II prodn. rate remained const.; av. II recovery was 78% and purity was 95%.

Enzymic production of 7-aminodeacetoxycephalosporanic acid

Enzymic production of 7-aminodeacetoxycephalosporanic acid. Machova, Jarmila; Vojtisek, Vladimir; Novak, Ludvik; Culik, Karel (Czech. ). Czech. CS 218023 B 15 Feb 1985,6 pp. (Czech). (Czechoslovakia). CODEN: CZXXA9. CLASS: IC: C12P035-00. APPLICATION: CS 81-6586 7 Sep 1981. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) Optimal conditions were studied for batchwise and continuous conversion of N-phenylacetamidodeacetoxycephalosporin (I) [27255-72-7] to the title compd. (II) [22252-43-3] by covalently crosslinked, permeabilized microbial cells having 2.5-8 units penicillin acylase [9014-06-6] activity/mg wet agent. Thus, 5 g of the biocatalyst was suspended in five 45-mL samples contg. an aq. I soln. in the concn. range of 1-10% and stirred at 37° and pH 7.6-8 (which was continuously adjusted with aq. NaOH). After 30-180 min, the biocatalyst was filtered, the samples were made acid with dil. H2SO4 to pH 4.2 at 4°, kept 1 h, and the cryst. products were sepd. to yield 80.6-86.2% II in 92-7% purity, depending on the reaction period and starting I concn.