Detail of "2731-73-9"

Reference

L-Cysteine

L-Cysteine. Yamada, Hideaki; Kumagai, Hidehiko; Ogishi, Haruyuki (Mitsubishi Chemical Industries Co., Ltd., Japan). Japan. Kokai JP 52136993 16 Nov 1977 Showa, 5 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12D013-06. APPLICATION: JP 76-52983 10 May 1976. DOCUMENT TYPE: Patent CA Section: 16 (Fermentations) A b-halogenoalanine and H2S or a thiol was reacted in the presence of 1 of a no. of yeast and bacterial species to prep. L-cysteine [52-90-4]. Thus, 1 mL cell ext. of Saccharomyces cerevisiae IFO 0334 was added to 10 mL of 0.04 M Tris-HCl buffer (pH 8.0) contg. b-chloro-L-alanine [2731-73-9] 500, Na2S 9H2O 500, and pyridoxal phosphate 1 m mole and reacted at 30° for 2 h. Prodn. of L-cysteine was 130 m mole.

L-Cysteine

L-Cysteine. Yamada, Hideaki; Kumagai, Hidehiko; Ookishi, Haruyuki (Mitsubishi Chemical Industries Co., Ltd., Japan). Japan. Kokai JP 51123887 28 Oct 1976 Showa, 5 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12D013-06. APPLICATION: JP 75-48440 21 Apr 1975. DOCUMENT TYPE: Patent CA Section: 16 (Fermentations) Cysteine [52-90-4] was produced by reaction of .beta.-substituted L-alanine and (NH4)2S [12135-76-1], (NH4)2Sx, or (NH4)HS in the presence of cysteine desulfhydrase [9012-96-8]. Thus, cells of Enterobacter aerogenes IFO 3320, which were cultured on a medium contg. L-cysteine 0.2, yeast ext. 0.5, meat ext. 0.5, polypeptone 0.5, glycerol 0.1, CaCl2 0.2, and NaCl 0.2% were added to 0.1 M ammonium buffer (pH 9.5) contg. .beta.-chloro-L-alanine [2731-73-9] 5 mM, (NH4)2S or (NH4)2Sx (S content = 5%) 0.5 mM, Na laurylsulfate 0.05%, and pyridoxal phosphate 0.1 mM. The mixt. was reacted at 30.degree. for 3 h. Prodn. of L-cysteine was 0.54 and 0.47 mM from (NH4)2S and (NH4)2Sx, resp.