Reference

Binding of metal ions by a-poly-L-lysine and e-poly-L-lysine

Binding of metal ions by a-poly-L-lysine and e-poly-L-lysine. Shima, Shoji; Sakai, Heiichi; Takagishi, Toru; Kuroki, Nobuhiko (Coll. Agric., Univ. Osaka Prefect., Sakai 591, Japan). J. Polym. Sci., Polym. Lett. Ed., 23(5), 245-9 (English) 1985. CODEN: JPYBAN. ISSN: 0360-6384. DOCUMENT TYPE: Journal CA Section: 36 (Physical Properties of Synthetic High Polymers) Section cross-reference(s): 34, 78 e-Poly-L-lysine (I) [28211-04-3] exhibited a pH-dependent binding affinity for Cu2+ which reached a max. at pH 4.9 and increased with increasing temp. Free energy, enthalpy, and entropy for the binding of Cu2+ by I were -2660 cal/mol, 5230 cal/mol, and 26.5 eu, resp., at 25°. I showed no binding ability for Co2+, Ni2+, and Zn2+, while a-poly-L-lysine [38000-06-5] had no observable binding properties with any cation. Absorbance measurements indicated that one Cu2+ ion is attached to one monomer residue. The large entropy gain in the Cu2+-I system resulted from the release of several hydrated water mols. accompanying the transfer of Cu2+ from aq. solvent to the polypeptide.

Purification and structural characterization of ePL produced by Kitasatospora sp

All Rights Reserved. Purification and structural characterization of ePL produced by Kitasatospora sp. PL6-3. Zhou, Jun; Xu, Hong; Wang, Jun; Yao, Zhong; Wang, Hui; Ouyang, Pingkai (Sch. Life Sci. Pharmaceutical Eng., Nanjing Univ. Technol., Nanjing, Jiangsu 210009, Peop. Rep. China). Huagong Xuebao (Chinese Edition), 57(8), 1957-1961 (Chinese) 2006 Huaxue Gongye Chubanshe. CODEN: HUKHAI. ISSN: 0438-1157. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) e-Poly-L-lysine (e-PL) was isolated and purified from the culture broth of Kitasatospora sp. PL6-3. Through the ion exchange process and Sephadex G-25 gel filtration chromatog., a high purity product was obtained with overall yield of 81.2%. The purified e-PL was characterized with UV, IR and HR/MAS NMR. 28211-04-3 which is the cas registry number of some chemical is mentioned. Its relative mol. wts. as detd. by SDS-PAGE and gel permeation chromatog. (GPC) were about 5.01 ′ 103 and 5.05 ′ 103 resp., which was higher than the other e-PL reported before. .