Detail of "28704-27-0"

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Effect of treatment with copolymer 1 (Cop-1) on the in vivo and in vitro manifestations of experimental allergic encephalomyelitis (EAE)

Effect of treatment with copolymer 1 (Cop-1) on the in vivo and in vitro manifestations of experimental allergic encephalomyelitis (EAE). Lisak, Robert P.; Zweiman, Burton; Blanchard, Nadine; Rorke, Lucy B. (Sch. Med., Univ. Pennsylvania, Philadelphia, PA, USA). J. Neurol. Sci., 62(1-3), 281-93 (English) 1983. CODEN: JNSCAG. ISSN: 0022-510X. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Copolymer 1 (Cop-1) [28704-27-0] treatment of guinea pigs (GP) sensitized with myelin basic protein (MBP) in adjuvant (20 mg/animal) lowers the incidence of clin. disease, decreases the severity of disease in affected GP, and has little effect on pathol. lesions. Lymphocytes of MBP-sensitized GP treated with Cop-1 exhibited in vitro proliferative responses to MBP equiv. to those of lymphocytes of untreated EAE GP. GP sensitized to MBP or Cop-1 (100 mg/animal) showed reactivity to the sensitizing antigen but little in vitro reactivity to the other antigen. There was no correlation between the in vitro lymphocyte response to MBP and Cop-1 in individual GP. Treatment of MBP-sensitized GP with calf thymus histone (CTH) also resulted in a lower incidence of clin. EAE with less severe disease in affected GP. There was little effect on the pathol. index and no evidence of either inhibition of MBP-induced lymphocyte proliferative responses or cross-reactivity between MBP and CTH. Thus, treatment with Cop-1 or CTH inhibits clin. manifestations of acute EAE without suppressing inflammatory cell infiltrates or sensitization to MBP.

The insulin receptor of rat brain is coupled to tyrosine kinase activity

The insulin receptor of rat brain is coupled to tyrosine kinase activity. Rees-Jones, Robert W.; Hendricks, S. Anne; Quarum, Merrit; Roth, Jesse (Diabetes Branch, Natl. Inst. Arthritis, Diabetes, Dig. Kidney Dis., Bethesda, MD 20205, USA). J. Biol. Chem.There are some reagents with their cas registry numbers 28704-27-0 and 31325-29-8 are used in this study., 259(6), 3470-4 (English) 1984. CODEN: JBCHA3. ISSN: 0021-9258. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Insulin [9004-10-8] receptors from rat brain were studied for receptor-assocd. tyrosine kinase [80449-02-1] activity. In solubilized, lectin-purified receptor prepns., insulin stimulated the phosphorylation of the b subunit of its receptor as well as of exogenous substrates. Phosphoamino acid anal. of casein phosphorylated by these prepns. revealed that 32P incorporation occurred predominantly on tyrosine residues. Receptor and casein phosphorylations were specific for insulin and analogs that also bind to the insulin receptor. The insulin dose response for phosphorylation of brain receptor resembled that reported for the purified insulin receptor from human placenta, suggesting similar insulin sensitivity and coupling of the brain receptor kinase. Four polyclonal antisera to the insulin receptor were able to bind and immunoppt. the brain receptor; however, only 2 antisera activated the receptor-assocd. kinase. Thus, the brain insulin receptor, like the well studied non-neural receptor, is coupled to tyrosine kinase activity, making possible regulation of cellular events by insulin in neural tissue. .