Reference

Freeze-drying of oxyhemoglobin: protection against oxidation in the presence of EDTA salts, sulfonic acid buffers, and pantothenic acid derivatives

Freeze-drying of oxyhemoglobin: protection against oxidation in the presence of EDTA salts, sulfonic acid buffers, and pantothenic acid derivatives. Fauvel, A.; Labrude, P.; Vigneron, C. (Lab. Hematol. Physiol., Fac. Sci., Pharm. Biol., Nancy 54001, Fr.). J. Pharm. Sci., 73(6), 784-7 (English) 1984. CODEN: JPMSAE. ISSN: 0022-3549. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Hb was freeze-dried in the presence of salts of EDTA, sulfonic acids used as buffers, or derivs. of pantothenic acid. At 0.In this experiment, several chemicals are used like 92471-58-4 25 M most of the compds. effectively inhibited the formation of metHb. The various model compds. used [Na Zn EDTA [14025-21-9], tris(hydroxymethyl)methylaminopropanesulfonic acid [29915-38-6], and DL-pantothenol [16485-10-2]] produced similar decreases in metHb formation as a function of the concn. of protective agent between 0.01 and 0.20 M. Expts. on the storage of the freeze-dried materials revealed substantial denaturation of oxyHb after 12 mo at 4° C. On the whole, these compds. were less effective than amino acid salts, during both desiccation and storage. The multiplicity of compds. that inhibit the denaturation of Hb during freeze-drying indicates that their mode of action is nonspecific. .

Methods and buffers for elution of DNA from paper for forensic analysis and diagnosis

Methods and buffers for elution of DNA from paper for forensic analysis and diagnosis. Davis, James C.; Hogan, Michael (Genvault Corporation, USA). PCT Int. Appl. WO 2005007852 A2 27 Jan 2005, 54 pp. DESIGNATED STATES: W: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, HR, HU, ID, IL, IN, IS, JP, KE, KG, KP, KR, KZ, LC, LK, LR, LS, LT, LU, LV, MA, MD, MG, MK, MN, MW, MX, MZ, NA, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RU, SC, SD, SE, SG, SK, SL, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, YU, ZA, ZM, ZW; RW: AT, BE, BF, BJ, CF, CG, CH, CI, CM, CY, DE, DK, ES, FI, FR, GA, GB, GR, IE, IT, LU, MC, ML, MR, NE, NL, PT, SE, SN, TD, TG, TR. (English). (World Intellectual Property Organization). CODEN: PIXXD2. CLASS: ICM: C12N015-10. APPLICATION: WO 2004-US22391 9 Jul 2004. PRIORITY: US 2003-2003/PV485972 9 Jul 2003. DOCUMENT TYPE: Patent CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 4, 9, 14 The present invention provides methods and buffers for elution of DNA from S&S 903, IsoCode, FTA paper for forensic anal. and diagnosis. The present invention provides a method for using an alk. elution buffer and mech. agitation to elute nucleic acids from dried samples on storage media at room temp.In this experiment, several chemicals are used like 828946-31-2 and 29915-38-6 In particular, the present invention provides a method for room temp. recovery of DNA, including double stranded DNA (dsDNA) and single stranded (ssDNA), from storage media. .