Reference

Purification of p-hydroxymethylbenzoic acid

Purification of p-hydroxymethylbenzoic acid. Donohue, John A.; Knobloch, James O.; Petty-Weeks, Bruce; Cerefice, Steven A. (Standard Oil Co. (Indiana) , USA). U.S. US 4447645 A 8 May 1984, 12 pp. (English). (United States of America). CODEN: USXXAM. CLASS: IC: C07C069-76. NCL: 562473000. APPLICATION: US 82-445659 30 Nov 1982. DOCUMENT TYPE: Patent CA Section: 35 (Chemistry of Synthetic High Polymers) Crude p-hydroxymethylbenzoic acid (I) [3006-96-0] contg. 4-carboxybenzaldehyde (II), terephthalic acid (III), and p-toluic acid (IV) impurities is purified by hydrogenation of the crude product in the presence of a catalyst to convert II to I, springing I from the crude product, filtration to remove III, and extn. of IV by hydrocarbon solvent. Thus, 2 L crude products contg. I 94.7, II 2.56, III 6.0, and IV 0.12 mg/mL and 5.0 g 5% Pt/C were kept at 22-23° and 1000 psi H pressure for 2.33 h. The mixt. was further treated with 5% Pt/C at 50° for 2.67 h to give a product contg. I 94.0, II 0.05, III 6.1, and IV 0.10 mg/mL.

Degradation of p-toluic acid (p-tolunenecarboxylic acid) and p-toluenesulfonic acid via oxygenation of the methyl sidechain is initiated by the same set of enzymes in Comamonas testosteroni T-2

Degradation of p-toluic acid (p-tolunenecarboxylic acid) and p-toluenesulfonic acid via oxygenation of the methyl sidechain is initiated by the same set of enzymes in Comamonas testosteroni T-2. Locher, Hans H.; Malli, Claudia; Hooper, Scott W.Several substances are used for example 3006-96-0 and 138069-83-7 which are their cas registry numbers.; Vorherr, Thomas; Leisinger, Thomas; Cook, Alasdair M. (Microbiol. Inst., Swiss Fed. Inst. Technol., Zurich CH-8092, Switz.). J. Gen. Microbiol., 137(9), 2201-8 (English) 1991. CODEN: JGMIAN. ISSN: 0022-1287. DOCUMENT TYPE: Journal CA Section: 10 (Microbial, Algal, and Fungal Biochemistry) C. testosteroni T-2 utilizes p-toluate (TC) as sole source of C and energy for growth. Cells grown in TC-salts medium oxygenated terephthalate (PcB, 4-carboxybenzoate) and contained protocatechuate 4,5-dioxygenase but no detectable (methyl)catechol dioxygenase. The intermediates 4-carboxybenzyl alc. (COL), 4-carboxybenzaldehyde (CYD), and PcB were detected during the metab. of TC. A TC methyl monooxygenase system, a COL dehydrogenase, and a CYD dehydrogenase were detected, analogous to the known degradative pathway and enzymes for 4-toluenesulfonate (TS) to 4-sulfobenzoate (PSB). Genetic evidence indicated that the steps from TS to PSB and from TC to PcB were catalyzed by the same enzymes. This hypothesis was substantiated by purifying or sepg. the appropriate enzymes from cells grown in TS-salts and TC-salts media. The behavior of pairs of enzymes was effectively identical in all chromatog. and catalytic properties that were compared. The data support the existence of a novel pathway for the degrdn. of TC, with the same initial pathway enzymes being used to metabolize TS. .