Detail of "30220-46-3"

Reference

Radioimmunoassay for phorbol esters using rabbit antiserums against phorbol succinate

Radioimmunoassay for phorbol esters using rabbit antiserums against phorbol succinate. Tashjian, Armen H., Jr.; Wolfson, Galina; Fearon, Clare W. (Lab. Toxicol., Harvard Sch. Public Health, Boston, MA 02115, USA). Cancer Res., 45(1), 103-7 (English) 1985. CODEN: CNREA8. ISSN: 0008-5472. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The phorbol nucleus was succinylated and then conjugated to bovine albumin using dicyclohexylcarbodiimide. Rabbits given injections of the conjugate developed antibodies which rose in titer progressively with repeated immunization. By the 9th bleeding, the binding of 1 antiserum, dild. 1:15,000, was satd. with ~10 nM 3H-labeled phorbol 12,13-dibutyrate (PDBU) [37558-16-0] and had an av. assocn. const., Ka, of 2.6 ′ 108M-1. The serol. specificity of the antisera was characterized by examg. the inhibition of the [3H]PDBU-anti-phorbol succinate immune system by 18 phorbol-related compds. The specificities of antibodies from 2 rabbits tested in detail were qual. similar. The rank order or inhibitory activity for certain phorbol-related compds. was PDBU [concn. of inhibitor required to give 50% inhibition of PDBU binding (IC50) = 7.6 nM] = phorbol 13-acetate [32752-29-7] (IC50 = 8.2 nM) > phorbol 12,13-dibenzoate [25405-85-0] > 4b-phorbol [17673-25-5] (IC50 = 124 nM) 3 phorbol 12,13-diacetate [24928-15-2] 3phorbol 12-myristate 13-acetate [16561-29-8] (IC50 = 2300 nM). The following compds. showed no detectable serol. activity: mezerein [34807-41-5], 4-O-methylphorbol 12-myristate 13-acetate [57716-89-9], ingenol [30220-46-3], 4a-phorbol [26241-63-4], teleocidin B [11032-05-6], and dihydroteleocidin B [7491-76-1]. Thus, the 4b-phorbol nucleus was required for serol. activity, esterification of the C-13 position with benzoate, acetate, or butyrate enhanced the immunoreactivity of 4b-phorbol, and among the phorbol-related compds. examd. there was no direct relation between serol. activity and biol. potency as tumor promoters. Using the [3H]PDBU-anti-phorbol succinate immune system, the concns. of immunoreactive phorbol-related material in crude mixts. such as croton oil were measured and pharmacokinetic studies in rats given PDBU s.c. were performed.

A method for the detection of diterpene ingenol by GLC

A method for the detection of diterpene ingenol by GLC. Upadhyay, R. R.; Bakhtavar (Fac. Pharm., Azarabadegan Univ., Tabriz, Iran). J. Indian Chem. Soc., 53(8), 864-5 (English) 1976. CODEN: JICSAH. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) A method for the detection of the diterpene ingenol (I) [30220-46-3] utilizes a cetylation of a I-contg. fraction from plant latex or plant exts., followed by gas chromatog. (GLC). Utilization of this method may make possible the identification of the substance responsible for tumor promotion and irritant activity in latex and plant exts. 30220-46-3 is just another one chemical used in this study. of Euphorbia species. .