Reference

The theaflavin monomers inhibit the cancer cells growth in vitro

The theaflavin monomers inhibit the cancer cells growth in vitro. Tu, You-Ying; Tang, An-Bin; Watanabe, Naoharu (Department of Tea Science, Zhejiang University, Hangzhou 310029, Peop. Rep. China). Acta Biochimica et Biophysica Sinica, 36(7), 508-512 (English) 2004 Shanghai Scientific and Technical Publishers. CODEN: ABBSC2. ISSN: 1672-9145. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The inhibition effects of tea theaflavins complex (TFs), theaflavin-3-3'-digallate (TFDG), theaflavin-3'-gallate (TF2B), and an unidentified compd. (UC) on the growth of human liver cancer BEL-7402 cells, gastric cancer MKN-28 cells and acute promyelocytic leukemia LH-60 cells were investigated.Some commonly used reagents like 28543-07-9 and 30462-35-2 are used in this experiment. TFs was obtained through the catalysis of catechins with immobilized polyphenols oxidase. TFDG, TF2B and UC were isolated from TFs with high speed countercurrent chromatog. (HSCCC). The results showed that TF2B significantly inhibited the growth of all three kinds of cancer cells, TFs, TFDG and UC had some effect on BEL-7402 and MKN-28, but little activity on LH-60. The inhibition effects of TF2B, TFDG, and UC on BEL-7402 and MKN-28 were stronger than TFs. The relationship coeffs. between monomer concn. and its inhibition rate against MKN-28 and BEL-7402 were 0.87 and 0.98 for TF2B, 0.96 and 0.98 for UC, resp. The IC50 values of TFs, TF2B, and TFDG were 0.18, 0.11, and 0.16 mM on BEL-7402 cells, and 1.11, 0.22, and 0.25 mM on MKN-28 cells resp. .