Detail of "30918-54-8"

Reference

Selectivity of the mutagenic action of some polynucleotides in Drosophila

Selectivity of the mutagenic action of some polynucleotides in Drosophila. Aleksandrov, Yu. N.; Gershenzon, S. M. (Inst. Mol. Biol. Genet., Kiev, USSR). Dopov. Akad. Nauk Ukr. RSR, Ser. B: Geol., Khim. Biol. Nauki, (7), 55-7 (Ukrainian) 1984. CODEN: DANND6. ISSN: 0377-9785. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Solns. of high-mol.-wt. synthetic polyriboadenylic acid [24937-83-5], polyriboinosylic acid [30918-54-8], polyriboadenylic cytidylic acid [55685-00-2] and polydeoxyadenylic acid [25191-20-2] injected into D. melanogaster males caused a 6-16-fold increase in the the total frequency of recessive lethals in the 2nd chromosome. The complementation anal. of the induced lethals showed an extremely high locus-specificity of these mutagens. Only a restricted no. of loci was affected with a frequency (per individual locus) ~1800-15,000-fold higher than in spontaneous mutations to recessive lethality.

Interaction of the 1a,25-dihydroxyvitamin D3 receptor with RNA and synthetic polyribonucleotides

Interaction of the 1a,25-dihydroxyvitamin D3 receptor with RNA and synthetic polyribonucleotides. Franceschi, Renny T. (Dep. Nutr., Harvard Sch. Public Health, Boston, MA 02115, USA). Proc. Natl. Acad. Sci. U. S. A., 81(8), 2337-41 (English) 1984. CODEN: PNASA6. ISSN: 0027-8424. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 6 The interaction of the 1a,25-dihydroxyvitamin D3 [32222-06-3] receptor with RNA and synthetic polynucleotides was examd. by using receptor from rachitic chicken intestine. Total intestinal RNA inhibited the binding of receptor to calf thymus DNA-cellulose with an efficiency equiv. to single-stranded DNA. A comparison of the inhibitory activity of several polyribonucleotides gave the following order of activities: poly(I) [30918-54-8] = poly(G) [25191-14-4] 3 double-stranded DNA > single-stranded DNA = poly(U) [27416-86-0]. Poly(A) [24937-83-5], poly(C) [30811-80-4], and the ribonucleoside monophosphates AMP, GMP, CMP, and UMP had minimal activity. A preference for single-stranded homopolymers was obsd. [i.e., poly(I) and poly(U) were active, whereas poly(I):poly(C) [24939-03-5] and poly(U):poly(A) [24936-38-7] were not]. The ability of nucleic acids to displace receptor from DNA-cellulose was also measured. Both poly(G) and poly(I) were more active than double-stranded DNA in this assay. Differences were noted between intestinal RNA fractions sepd. on the basis of poly(A) content. Receptor also bound to immobilized intestinal RNA and polynucleotides. The KCl concn. necessary to disrupt binding to a given polynucleotide generally paralleled the activity of that mol. in DNA-cellulose inhibition and displacement assays. Apparently, the 1a,25-dihydroxyvitamin D3 receptor can interact with RNA as well as DNA.