Reference

Expression in Streptomyces ambofaciens of an Escherichia coli K-12 gene which confers resistance to hygromycin B

Expression in Streptomyces ambofaciens of an Escherichia coli K-12 gene which confers resistance to hygromycin B. Kuhstoss, S.; Rao, R. Nagaraja (Lilly Res. Lab., Indianapolis, IN 46285, USA). Gene, 26(2-3), 295-9 (English) 1983. CODEN: GENED6. ISSN: 0378-1119. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Two plasmid vectors (pKC293 and pKC305) were constructed that can replicate in E. coli K-12 and S. 1404-04-2 and 77000-12-5 which are cas registry numbers of chemicals are mentioned. ambofaciens. These shuttle vectors were used to demonstrate the expression of 2 E. coli genes, hygromycin B [31282-04-9] resistance and transposon Tn5 neomycin [1404-04-2] resistance, in S. ambofaciens. .

The in vitro activity of various anthelmintic compounds against Haemonchus contortus larvae

The in vitro activity of various anthelmintic compounds against Haemonchus contortus larvae.Some chemicals with cas registry numbers like 14769-73-4 and 7181-73-9 are also used. Boisvenue, R. J.; Brandt, M. C.; Galloway, R. B.; Hendrix, J. C. (Parasitol. Dep., Eli Lilly and Co., Greenfield, IN 46140, USA). Vet. Parasitol., 13(4), 341-7 (English) 1983. CODEN: VPARDI. ISSN: 0304-4017. DOCUMENT TYPE: Journal CA Section: 5 (Agrochemical Bioregulators) Twenty-five known anthelmintic compds. were evaluated in vitro against the highly motile exsheathed nonfeeding 3rd-stage of H. contortus larvae. Activity was based on lack of motility or death of larvae after 24 h of chem. exposure. Six compds. (avermectin [73989-17-0], closantel [57808-65-8], levamisole [14769-73-4], morantel [20574-50-9], phenylhydrazine [100-63-0], and ticarbodine [31932-09-9]) were active at a concn. of ￡100 mg/cm3. The most active compds. were avermectin and levamisole. When higher in vitro concns. were used, 10 compds. (bephenium [7181-73-9], coumaphos [56-72-4], dichlorovos [62-73-7], disophenol [305-85-1], hygromycin b [31282-04-9], methyridine [114-91-0], parbendazole [14255-87-9], phenothiazine [92-84-2], pyrantel [15686-83-6], and thiabendazole [148-79-8]) exhibited activity. Nine compds. were inactive; among these were the new benzimidazoles, i.e., albendazole, fenbendazole, mebendazole, and oxibendazole. Because of the inactivity of the new benzimidazoles, this in vitro system is unsuitable as a routine screening tool. Also, the system favors drugs that act quickly through percuticular entry. In an initial group of 5280 untested compds., 254 (4.8%) exhibited in vitro activity at 100 mg/cm3 against the nonfeeding larvae stage. The exogenous and in vitro cultivation techniques required for collecting, cleaning and exsheathing the larvae are described. .