Detail of "3310-41-6"

Reference

Mutagenicity of N4-aminocytidine and its derivatives in Chinese hamster lung V-79 cells

Mutagenicity of N4-aminocytidine and its derivatives in Chinese hamster lung V-79 cells. Incorporation of N4-aminocytosine into cellular DNA. Nomura, Akinori; Negishi, Kazuo; Hayatsu, Hikoya; Kuroda, Yukiaki (Fac. Pharm. Sci., Okayama Univ., Okayama 700, Japan). Mutat. Res., 177(2), 283-7 (English) 1987. CODEN: MUREAV. ISSN: 0027-5107. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) N4-Aminocytidine (I) [57294-74-3] induced mutation to 6-thioguanine [154-42-7] resistance in Chinese hamster lung V-79 cells in culture. Previous studies with exptl. systems of in vitro DNA synthesis and of phage and bacterial mutagenesis have shown that this nucleoside analog induced base-pair transitions through its incorporation into DNA, with its erroneous base-pairing property. Incorporation of exogenously added [5-3H]N4-aminocytidine into the DNA of V-79 cells was obsd. in the present study. N4-Aminodeoxycytidine [52725-05-0] was not mutagenic for the V-79 cells. Several alkylated N4-aminocytidine derivs. were tested for their mutagenicity in this system. Those with an alkyl group on the N'-nitrogen of the hydrazino group at position 4 of N4-aminocytidine were mutagenic, but those having an alkyl on the N4-nitrogen were not. These results are consistent with those previously obsd. in the bacterial mutagenesis systems, and agree with a mechanism of mutation in which a tautomerization of N4-aminocytosine [3310-41-6] is the necessary step for causing the erroneous base pairing.Except for chemicals metioned above, 52725-05-0 and 3310-41-6 are also used. .

Proofreading of a mutagenic nucleotide, N4-aminodeoxycytidylic acid, by Escherichia coli DNA polymerase I

Proofreading of a mutagenic nucleotide, N4-aminodeoxycytidylic acid, by Escherichia coli DNA polymerase I. Takahashi, Mitsuko; Negishi, Kazuo; Hayatsu, Hikoya (Fac. Pharm. Sci., Okayama Univ., Okayama 700, Japan). Biochem. Biophys. Res. Commun., 143(1), 104-9 (English) 1987. CODEN: BBRCA9. ISSN: 0006-291X. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) N4-aminodeoxycytidine triphosphate [90335-46-9], a putative metabolite of N4-aminocytidine [52725-05-0] which is a potent mutagen, is incorporated in vitro into polynucleotides in place of dCTP and at a much lesser extent, but significantly, in place of dTTP by the E. coli DNA polymerase I [9012-90-2] large fragment. The activity of the polymerase to proofread this unnatural nucleotide was investigated. The 3'-5' exonuclease [79393-91-2] in the polymerase recognizes N4-aminocytosine [3310-41-6] as an incorrect base when N4-aminocytosine is incorporated opposite adenine, but the enzyme cannot distinguish N4-aminocytosine from cytosine when it is incorporated opposite guanine.Except for chemicals metioned above, 3310-41-6 and 79393-91-2 are also used. .