Reference

GLC determination of free and conjugated triclosan in human plasma and urine

GLC determination of free and conjugated triclosan in human plasma and urine. Sioufi, Antoine; Caudal, Francoise; Grimault, Danielle (Ciba-Geigy Biopharm. Res. Cent., Rueil-Malmaison, Fr.). J. Pharm. Sci., 66(8), 1166-8 (English) 1977. CODEN: JPMSAE. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) A gas-liq. chromatog. (GLC) method for the detn. 64595-63-7 is the cas registry number of certain chemical which is used as reagents here. of free and conjugated triclosan (I) [3380-34-5] at concns. as low as 2 ng/mL in human plasma or urine is described. Conjugated metabolites are split by enzyme hydrolysis. After addn. of an internal std., I is extd. at acid pH into petroleum ether, transferred to an alk. aq. soln., and back-extd. into petroleum ether after acidification. Both compds. are acetylated with acetic anhydride in the presence of pyridine. The acetyl derivs. are detd. by GLC using a 63-Ni-electron-capture detector. .

The determination of Irgasan DP300 in biological fluids

The determination of Irgasan DP300 in biological fluids. Hoar, D. R.; Sissons, D. J. (Colworth/Welwyn/Res. Lab., Unilever, Sharnbrook/Bedford, Engl.). Methodol. Dev. Biochem., 5, 221-6 (English) 1976. CODEN: MDBCAU. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) A gas chromatog. method is presented for the detn. of Irgasan DP 300 (2-hydroxy-2',4,4'-trichlorodiphenyl ether)(I) [3380-34-5] in blood, urine, bile, etc. The method is capable of detecting 10 .mu.g I/L. In comparison with enzymic hydrolysis, acid hydrolysis of the conjugate is an improvement with respect to speed and effectiveness. Radiochem. checking shows reproducible recoveries, 95-105% for free I and 80-95% for Irgasan DP 300 glucuronide [63156-12-7].