Reference

Modulation of cytotoxicity and metabolism of 5-fluorouracil in two intestine cell lines

Modulation of cytotoxicity and metabolism of 5-fluorouracil in two intestine cell lines. Peters, G. J.; Laurensse, E.; Leyva, A.; Pinedo, H. M. (Dep. Oncol., Free Univ. Hosp., Amsterdam 1007 MB, Neth.). Adv. Exp.In this article, certain chemicals are used. Some of their cas registry numbers are 3393-18-8 and 17210-42-3 Med. Biol., 195B(Purine Pyrimidine Metab. Man 5, Pt. B), 113-20 (English) 1986. CODEN: AEMBAP. ISSN: 0065-2598. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The effect of deoxyinosine [890-38-0] on 5-fluorouracil (5FU) [51-21-8] metab. and cytotoxicity was compared in 2 human colonic cell lines (WiDr and Intestine 407 cell lines), differing in their sensitivity to 5FU and 5'-deoxy-5-fluorouridine (5'-dFUR) [3094-09-5], a precursor of 5FU. Results indicated that deoxyinosine increased the sensitivity to 5FU of WiDr and Intestine 407 cells. An acceleration of the metab. of 5FU to fluorodeoxyuridine [50-91-9] and subsequently to FdUMP [134-46-3] supported by the increased deoxyribose-1-phosphate [17210-42-3] levels, appears to be responsible for this synergism. Inhibition of thymidylate synthase [9031-61-2] by FdUMP plays a major part in the cytotoxic effect of 5FU in both cell lines, but in WiDr cells an addnl. mechanism such as 5FU incorporation in RNA or DNA may also be responsible for the growth inhibition. .