Reference

A comparative study of the cytotoxic effect of dammaran row triterpenoids and betulin on early embryogenesis of the sea urchin

A comparative study of the cytotoxic effect of dammaran row triterpenoids and betulin on early embryogenesis of the sea urchin. Anisimov, M. M.; Shentsova, E. B.; Shcheglov, V. V.; Strigina, L. I.; Uvarova, N. I.; Levina, E. V.; Oshitok, G. I.; Elyakov, G. B. (Far East Sci. Cent., Pac. Inst. Bio-Org. Chem., Vladivostock, USSR). Toxicon, 16(1), 31-6 (English) 1978. CODEN: TOXIA6. ISSN: 0041-0101. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The relation between chem. structure and cytotoxic effect of 35 triterpenes and of their dammaran row glycosides and betulin [473-98-3] was studied. Some of these compds. had a cytotoxic effect on developing sea urchin embryos. Depending on their structure and concn., the glycosides caused anomalies in embryo development, arrest of egg division, and blastomere lysis. Panaxoside A aglycone Af [34080-08-5], dihydropanaxoside A aglycone [19536-31-3], and panaxadiol diglucoside [60431-54-1] had the highest cytostatic activity (10, 12, and 25 mg/mL, resp.). Betulin glycosides (100 mg/mL) have no cytostatic effect on developing sea urchin embryos.

Spectrodensitometric TLC scanning in the determination of Sichuan Zhu Jie ginseng sapogenins

Spectrodensitometric TLC scanning in the determination of Sichuan Zhu Jie ginseng sapogenins. Cai, Ping; Qiu, Xiaoxing; Xiao, Zhuoyin (Inst. Meter. Med., Sichuan Med. Coll., Chengdu, Peop. Rep. China). Sichuan Yixueyuan Xuebao, 15(3), 237-42 (Chinese) 1984. CODEN: CIYPDA. ISSN: 0253-4290. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Section cross-reference(s): 63 Sapogenins were isolated from saponins of Zhu-jie ginseng (root of Panax japonicus) grown in Sichuan, China, by H2SO4-EtOH hydrolysis, filtration, CHCl3 extn., and silica gel column chromatog. They were then dissolved in CHCl3-abs. EtOH on silica gel plates with either C6H6-Me2CO (8:2) or C6H6-Et2O (1:1). Plates were then soaked in NH4HSO4 and heated at 105-110° for 12 min for color development. Oleanolic acid (I) [508-02-1] was detd. by densitometry at 525 nm and 20S-protopanaxadiol (II) [30636-90-9], 20S-protopanaxatriol (III) [34080-08-5], and 3b,6a,12b,25-tetrahydroxy-(20d,24R)-epoxydammarane (IV) [69926-31-4] at 400 nm. The sapogenins of Sichuan ginseng were: I 90.5, II 2.4, III 5.2, and IV 1.8%. Differences in the relative amts. of the sapogenins found in Japanese Zhu-jie ginseng are discussed.