Detail of "34317-61-8"

Reference

S-Phenyl-L-cysteine production process

S-Phenyl-L-cysteine production process. Fukuta, Kazuhiro; Fukuhara, Nobuhiro (Mitsui Toatsu Chemicals, Incorporated, Japan). Eur. Pat. Appl. EP 754759 A1 22 Jan 1997, 13 pp. 34317-61-8 and 56-45-1 are also occured in this study. DESIGNATED STATES: R: CH, DE, FR, GB, IT, LI. (English). (European Patent Organization). CODEN: EPXXDW. CLASS: ICM: C12P013-12. APPLICATION: EP 1996-305172 15 Jul 1996. PRIORITY: JP 1995-181537 18 Jul 1995. DOCUMENT TYPE: Patent CA Section: 16 (Fermentation and Bioindustrial Chemistry) S-Phenyl-L-cysteine (I) is produced in high yield by reacting thiophenol and L-serine under the action of tryptophan synthase at a pH range of 9.0-10.5. Purifn. of I obtained by this enzyme reaction can be effectively achieved by adjusting the pH of a crystal-contg. reaction mixt. to a strongly acidic level of ￡1.5 to dissolve crystals of I, adding activated C to the pH-adjusted mixt., maintaining the resultant mixt. at a temp. of 20-60° under aeration with an O2-contg. gas, subjecting the thus-obtained mixt. to filtration, raising the pH of the resulting filtrate back to a range of 2.5-6.0 to ppt. crystals of I, and then recovering the thus-pptd. crystals. .

S-Phenylcysteine in albumin as a benzene biomarker

S-Phenylcysteine in albumin as a benzene biomarker. Bechtold, William E.; Strunk, Michael R. (Inhalation Toxicology Research Institute, Albuquerque, NM, USA). Environmental Health Perspectives Supplements, 104(6, Benzene Toxicity, Carcinogenesis, and Epidemiology), 1147-1149 (English) 1996 National Institute of Environmental Health Sciences. CODEN: EHPSEO. ISSN: 1078-0475. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) Section cross-reference(s): 59 Previous results from our lab. have shown that benzene is metabolized by humans to form the adduct S-phenylcysteine (SPC). Levels of SPC measured in humans occupationally exposed to benzene were increased linearly relative to exposure concns. ranging from 0 to 23.1 ppm for 8 h/day, 5 days/wk. However, the method of measurement used was laborious, prone to imprecision and interferences, and insufficiently sensitive for the low-dose exposures anticipated in the United States (100 ppb>). An improved chem. method was necessary before SPC adducts in albumin could be used as a benzene biomarker. A simple, sensitive method to measure SPC adducts is being developed and is based on the cleavage of the cysteine sulfhydryl from blood proteins treated with Raney nickel (RN) in deuterium oxide. The product of the reaction with SPC is monodeuterobenzene. SPC treated with RN released monodeuterobenzene in a concn.-dependent fashion. SPC was measured by RN treatment of globin from rats repeatedly exposed by inhalation to 600 ppm benzene. SPC levels measured using the RN approach were 690±390 pmol SPC/mg Hb (mean ± % difference), as opposed to 290±45 pmol SPC/mg Hb as measured by our previous method. This method may facilitate the cost-effective, routine anal. of SPC in large populations of people exposed to ambient levels of benzene.Except for chemicals metioned above, 71-43-2 and 34317-61-8 are also used. .