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Highly stable phospholipid unilamellar vesicles from spontaneous vesiculation: a DLS and SANS study

Highly stable phospholipid unilamellar vesicles from spontaneous vesiculation: a DLS and SANS study. Yue, Baohua; Huang, Chien-Yueh; Nieh, Mu-Ping; Glinka, Charles J.; Katsaras, John (Otto H. York Department of Chemical Engineering and Department of Chemistry and Environmental Science, New Jersey Institute of Technology, Newark, NJ 07102, USA). Journal of Physical Chemistry B, 109(1), 609-616 (English) 2005 American Chemical Society. CODEN: JPCBFK.Chemicals with cas numbers 34506-67-7 and 18194-24-6 also play role. ISSN: 1520-6106. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) Spontaneously formed unilamellar vesicles (ULV) composed of short- and long-chain phospholipids, dihexanoyl phosphorylcholine (DHPC) and dimyristoyl phosphorylcholine (DMPC), resp., were doped with a neg. charged lipid, dimyristoyl phosphorylglycerol (DMPG), and studied with small-angle neutron scattering (SANS) and dynamic light scattering (DLS). Upon diln., the spontaneous formation of vesicles was found to take place from bilayered micelles, or so-called "bicelles". SANS and DLS data show that ULV with narrow size distributions are highly stable at low lipid (Clp < 0.50 wt %) and NaCl salt (Cs) concns. ULV size was found to be independent of both Clp and Cs when they were below 0.33 and 0.5 wt %, resp. Surface charge and salinity were found to be important factors in prepg. ULV of a certain size. This observation is not in complete agreement with previous exptl. results and cannot be completely explained with current theor. predictions based on equil. calcns. for catanionic surfactant mixts. ULV size is found to be invariant over a wide range of temps., both below and above the phase-transition temp., TM, of DMPC, and was stable for periods of weeks and months, even after sonication. .

Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles

Stereospecific assignments of the isopropyl methyl groups of the membrane protein OmpX in DHPC micelles. Hilty, Christian; Wider, Gerhard; Fernandez, Cesar; Wuethrich, Kurt ( Institut fuer Molekularbiologie und Biophysik, Eidgenoessische Technische Hochschule Zuerich, Zurich CH-8093, Switz.). Journal of Biomolecular NMR, 27(4), 377-382 (English) 2003 Kluwer Academic Publishers. CODEN: JBNME9. ISSN: 0925-2738. DOCUMENT TYPE: Journal CA Section: 6 (General Biochemistry) Section cross-reference(s): 77 In NMR studies of large mol.Several reagents such as 34506-67-7 is used here. structures, the no. of conformational constraints based on NOE measurements is typically limited due to the need for partial deuteration. As a consequence, when using selective protonation of peripheral Me groups on a perdeuterated background, stereospecific assignments of the diastereotopic Me groups of Val and Leu can have a particularly large impact on the quality of the NMR structure detn. For example, 3D 15N- and 13C-resolved [1H,1H]-NOESY spectra of the E. Coli membrane protein OmpX in mixed micelles with DHPC, which have an overall mol. wt. of about 60 kDa, showed that about 50% of all obtainable NOEs involve the diastereotopic Me groups of Val and Leu. In this paper, we used biosynthetically-directed fractional 13C labeling of OmpX and [13C,1H]-HSQC spectroscopy to obtain stereospecific Me assignments of Val and Leu in OmpX/DHPC. For practical purposes it is of interest that this data could be obtained without use of a deuterated background, and that combinations of NMR expts. have been found for obtaining the desired information either at a 1H frequency of 500 MHz, or with significantly reduced measuring time on a high-frequency instrument. .