Reference

Test implement and test method for colorimetrically determining whether a female is fertile or pregnant

Test implement and test method for colorimetrically determining whether a female is fertile or pregnant. Manautou, Jorge M.; Garcia, Adolfo R. (Alza Corp., USA). Can. CA 1032452 6 Jun 1978, 12 pp. (English). (Canada). CODEN: CAXXA4. NCL: 150-10. APPLICATION: CA 75-223218 27 Mar 1975. DOCUMENT TYPE: Patent CA Section: 63 (Pharmaceuticals) A test implement for detg. female fertility or pregnancy colorimetrically dets. N-acetyl-b-glucosaminidase [9012-33-3] in the female saliva. It comprises an absorbent material contg. a buffer and an N-acetyl-b-d-glucosamidine which reacts with the enzyme to form a phenol with distinct color. Thus, a test implement was prepd. by impregnating a paper strip with p-nitrophenyl-N-acetyl-b-d-glucosamidine [3459-18-5] in pH 4-5 0.1M citrate buffer and drying. To detect fertility the strip was wetted with the subject's saliva and allowed to stand ~30 min. The phenol reaction product was developed by wetting the strip with pH 9-11 0.1M Na glycinate buffer and the color was compared with a std. color chart. The test inplement shows a distinct color change during a period from about 18 to 11 days prior to menstruation. This test is more sensitive and accurate than the alk. phosphatase test.

b-N-Acetylhexosaminidase in human neoplasia and in regenerating planaria Dugesia lugubris: kinetic studies

b-N-Acetylhexosaminidase in human neoplasia and in regenerating planaria Dugesia lugubris: kinetic studies. Orlacchio, Aldo; Principato, Giovanni B.; Maffei, Claudio; Montanini, Isabella; Giovannini, Elvio (Fac. Sci., Univ. Perugia, Perugia 06100, Italy). Biochem. Int.Several reagents with their cas registry numbers 14948-96-0 and 3459-18-5 are used here., 7(4), 501-10 (English) 1983. CODEN: BIINDF. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) b-N-Acetylhexosaminidase (I) from human neoplastic colon, regenerating planaria, and relative control tissues were partially purified by means of affinity chromatog. on concanavalin A-Sepharose. The I activities in proliferating tissues were in higher concn. than in control tissues, but they showed the same kinetic consts. I from human source hydrolyzed p-nitrophenyl-N-acetyl-b-D-galactosaminide, whereas the I from planaria hydrolyzed both substrates with the same Vmax. Acetate, acetamide, a-methylmannoside, and N-acetylglucosamine were competitive inhibitors of the studied enzymes. N-Acetylgalactosamine was the strongest inhibitor, competitive with I from planaria, and of the mixed-type with human I. .