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| CAS No.: | 3483-12-3 |
|---|---|
| Name: | DL-1,4-Dithiothreitol |
| Article Data: | 12 |
| Molecular Structure: | |
|
|
|
| Formula: | C4H10O2S2 |
| Molecular Weight: | 154.254 |
| Synonyms: | 2,3-Butanediol,1,4-dimercapto-, (R*,R*)-;Threitol, 1,4-dithio- (7CI,8CI);1,4-Dithio-DL-threitol;1,4-Dithiothreitol;Cleland's reagent;DL-1,4-Dimercapto-2,3-dihydroxybutane;Dithiothreitol;DTT (threitol derivative);Reagents, Cleland's;Sputolysin;WR 34678;rac-Dithiothreitol;threo-1,4-Dimercapto-2,3-butanediol;threo-2,3-Dihydroxy-1,4-butanedithiol;threo-2,3-Dihydroxy-1,4-dithiolbutane;2,3-Butanediol,1,4-dimercapto-, (2R,3R)-rel-; |
| EINECS: | 222-468-7 |
| Density: | 1.303 g/cm3 |
| Melting Point: | 41-44 °C(lit.) |
| Boiling Point: | 364.453 °C at 760 mmHg |
| Flash Point: | 174.215 °C |
| Solubility: | Freely soluble in water |
| Appearance: | White crystalline powder |
| Hazard Symbols: |
 Xn
|
| Risk Codes: | 25-36/37/38-22-20/21/22 |
| Safety: | 26-45-37/39-36 |
| PSA: | 118.06000 |
| LogP: | -0.43220 |
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Chemistry
Molecular Structure of Dithiothreitol (CAS NO.3483-12-3):
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Molecular Formula: C4H10O2S2
Molecular Weight: 154.251
IUPAC Name: 1,4-Bis(sulfanyl)butane-2,3-diol
CAS NO: 3483-12-3
Classification Code: Drug / Therapeutic Agent ; Mutation data
Melting point: 41-44 °C
Index of Refraction: 1.579
Molar Refractivity: 39.36 cm3
Molar Volume: 118.4 cm3
Surface Tension: 55.5 dyne/cm
Density: 1.302 g/cm3
Flash Point: 174.2 °C
Enthalpy of Vaporization: 70.67 kJ/mol
Boiling Point: 364.5 °C at 760 mmHg
Vapour Pressure: 8.68E-07 mmHg at 25 °C
EINECS: 222-468-7
Product Categories: Chemistry
storage temp.: 2-8 °C
solubility: H2O: 50 mg/mL, clear, colorless
Water Solubility: freely soluble
Sensitive: Air Sensitive
Stability: Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 °C.
Appearance: White solid
Uses
A common use of Dithiothreitol (CAS NO.3483-12-3) is as a reducing or "deprotecting" agent for thiolated DNA. It is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. It can also be used as an oxidizing agent.
Toxicity Data With Reference
| Organism | Test Type | Route | Reported Dose (Normalized Dose) | Effect | Source |
|---|---|---|---|---|---|
| mouse | LD50 | intramuscular | 108mg/kg (108mg/kg) | BEHAVIORAL: CONVULSIONS OR EFFECT ON SEIZURE THRESHOLD | Journal of Pharmacy and Pharmacology. Vol. 1, Pg. 576, 1949. |
| mouse | LD50 | intraperitoneal | 154mg/kg (154mg/kg) | Yakugaku Zasshi. Journal of Pharmacy. Vol. 94, Pg. 1419, 1974. |
Safety Profile
Hazard Codes of Dithiothreitol (CAS NO.3483-12-3): 
Xn
Risk Statements: 25-36/37/38-20/21/22
R25: Toxic if swallowed.
R36/37/38: Irritating to eyes, respiratory system and skin.
R20/21/22: Harmful by inhalation, in contact with skin and if swallowed.
Safety Statements: 26-45-37/39-36
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S45: In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S37/39: Wear suitable gloves and eye/face protection.
S36: Wear suitable protective clothing.
WGK Germany: 3
RTECS: EK1610000
F: 3-10-16-23
Poison by subcutaneous, intravenous, and intraperitoneal routes. Mutation data reported. When heated to decomposition it emits toxic fumes of SOx.
Specification
Dithiothreitol (CAS NO.3483-12-3), its Synonyms are (R*,R*)-(+-)-1,4-Dimercapto-2,3-butanediol ; 1,4-Dithiothreitol ; 2,3-Butanediol, 1,4-dimercapto-, DL-threo- ; Cleland reagent ; Cleland's reagent ; D-1,4-Dithiothreitol ; D-threo-1,4-Dimercapto-2,3-butanediol ; DL-threo-1,4-Dimercapto-2,3-butanediol ; Sputolysin ; Threitol, 1,4-dithio- ; (R*,R*)-(1)-1,4-Dimercaptobutane-2,3-diol ; (R*,R*)-1,4-Dimercaptobutane-2,3-diol ; 2,3-Butanediol, 1,4-dimercapto-, (2R,3R)-rel- ; 2,3-Butanediol, 1,4-dimercapto-, (R*,R*)- (+-)- (9CI) ; 2,3-Butanediol, 1,4-dimercapto-, (theta,theta)-(+/-)- ; 2,3-Butanediol, 1,4-dimercapto-, D-threo- . Due to air oxidation, Dithiothreitol is a relatively unstable compound whose useful life can be extended by refrigeration and handling in an inert atmosphere. Since protonated sulfurs have lowered nucleophilicities, it becomes less potent as the pH lowers. Tris(2-carboxyethyl)phosphine HCl (TCEP hydrochloride) is an alternative which is more stable and works even at low pH.