Reference

The metabolic fate of 2H-labeled propafenone in man

The metabolic fate of 2H-labeled propafenone in man. Hege, H. G.; Hollmann, M.; Kaumeier, S.; Lietz, H. (Biochem. Hum. Pharmacol. Dep., Knoll A.-G., Ludwigshafen/Rhein. 6700, Fed. Rep. Ger.). Eur. J. Drug Metab. Pharmacokinet., 9(1), 41-55 (English) 1984. CODEN: EJDPD2. ISSN: 0398-7639. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The metab. of propafenone (I) [54063-53-5] was studied in 3 humans by using 300 mg of the deuterated compd. given orally. Propafenone is absorbed completely and quant. metabolized. Unchanged propafenone is excreted neither in the urine nor in the feces in amt. of >1% of the dose. The percentage of free unconjugated propafenone of the total deuterium content in the plasma was about 10% 3 and 6 h after application. Conjugates of hydroxylated derivs. of propafenone are present predominantly in the plasma. The excretion of the metabolites takes place mainly by way of the feces, 53% of the dose is excreted by this route within 48 h. Urinary elimination accounted for 18.Several substances like 54063-53-5 may be metioned in this study.5 and 38% of the dose within 48 h in 2 humans. It was possible to elucidate the structure of 11 metabolites, accounting for more than 90% of the dose administered. The major metabolites are conjugates of 5-hydroxypropafenone and hydroxymethoxypropafenone with glucuronic and sulfuric acid and propafenone glucuronide [91411-76-6]. Furthermore, metabolic products of oxidative deamination pathway were identified, i.e. a glycol [91401-73-9] and a lactic acid deriv. [91401-74-0]. C-C splitting yields a relatively large amt. of 3-phenylpropionic acid [501-52-0], while cleavage of the ether group leads to a phenolic product [3516-95-8] and is only of minor importance. .

Chalcone dimethylallyltransferase from Morus nigra cell cultures

Chalcone dimethylallyltransferase from Morus nigra cell cultures. Substrate specificity studies. Vitali, Alberto; Giardina, Bruno; Delle Monache, Giuliano; Rocca, Filippo; Silvestrini, Andrea; Tafi, Andrea; Botta, Bruno (Istituto di Biochimica e Biochimica Clinica, Sezione di Roma, Istituto di Chimica del Riconoscimento Molecolare, Universita Cattolica del S. Cuore, Rome 00168, Italy). FEBS Letters, 557(1-3), 33-38 (English) 2004 Elsevier Science B.V.Chemical with cas number 3516-95-8 also plays role. CODEN: FEBLAL. ISSN: 0014-5793. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) A new prenyltransferase (PT) enzyme derived from the microsomal fractions of cell cultures of Morus nigra was shown to be able to prenylate exclusively chalcones with a 2',4'-dihydroxy substitution and the isoflavone genistein. Computational studies were performed to shed some light on the relationship between the structure of the substrate and the enzymic activity. PT requires divalent cations, particularly Mg2+, to be effective. The apparent Km values for g,g-dimethylallyldiphosphate and 2',4'-dihydroxychalcone were 63 and 142 mM, resp. The max. activity of the enzyme was expressed during the first 10 days of cell growth. .