Detail of "35495-11-5"

Reference

Dependence on exogenous metabolic activation for induction of unscheduled DNA synthesis in Syrian hamster embryo cells by diethylstilbestrol and related compounds

Dependence on exogenous metabolic activation for induction of unscheduled DNA synthesis in Syrian hamster embryo cells by diethylstilbestrol and related compounds. Tsutsui, Takeki; Degen, Gisela H.; Schiffmann, Dietmar; Wong, Annette; Maizumi, Heiji; McLachlan, John A.; Barrett, J. Carl (Natl. Inst. Environ. Health Sci., Research Triangle Park, NC 27709, USA). Cancer Res., 44(1), 184-9 (English) 1984. CODEN: CNREA8. ISSN: 0008-5472. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Whether DES (I) [56-53-1] induced unscheduled DNA synthesis (UDS) in Syrian hamster embryo cells under the conditions which resulted in cell transformation and the role of an exogenous metabolic activation system on DES-induced UDS were studied. DES (1-10 mg/mL) failed to induce any detectable UDS in the cells, whereas other known transforming agents, including UV irradn. (6-24 J/m2), benzo[a]pyrene [50-32-8] (0.Several reagents with their cas registry numbers 70-70-2 and 22610-99-7 are used here.1-1.0 mg/mL), and aflatoxin B1 [1162-65-8] (10-100 mg/mL) induced significant levels of UDS. In contrast, UDS was induced in a dose-dependent manner by DES after addn. of an Aroclor-induced rat liver postmitochondrial supernatant fraction and other cofactors for exogenous metabolic activation. The ability of structural analogs and metabolites of DES to induce UDS was examd. In the absence of exogenous activation, the only oxidative metabolite of DES detected in the presence of the cells was cis,cis-dienestrol [35495-11-5] which did not induce UDS by itself. In the presence of exogenous activation, cis,cis-dienestrol and trans,trans-dienestrol [13029-44-2] induced UDS, but not to a greater extent than DES. With the exogenous metabolizing system, increased metab. of DES to cis,cis-dienestrol and addnl. polar derivs. of DES or dienestrol, possibly hydroxylated derivs., were obsd. With exogenous metabolic activation, 3,5,3',5'-tetrafluorodiethylstilbestrol [71292-84-7] and hexestrol [84-16-2], which differ in their ability to be peroxidatively metabolized both induced UDS. None of the DES related compds. examd. was active in the UDS assay without exogenous metabolic activation, but all of the compds. can potentially form phenoxy radical intermediates by a peroxidase-mediated reaction. The compds. which can be further oxidized to a quinone were most active in inducing UDS. .

Influence of vitamin C on estrogen-induced renal carcinogenesis in Syrian hamster

Influence of vitamin C on estrogen-induced renal carcinogenesis in Syrian hamster.Some chemicals with cas registry numbers like 50-81-7 and 56-53-1 are also used. Liehr, Joachim G.; Wheeler, William J.; Ballatore, Annie M. (Med. Sch., Univ. Texas, Houston, TX 77025, USA). Modulation Mediation Cancer Vitam., [Proc. - Int. Conf.], 1st, Meeting Date 1982, 132-9. Edited by: Meyskens, Frank L.; Prasad, Kedar N. Karger: Basel, Switz. (English) 1983. CODEN: 51AEAG. DOCUMENT TYPE: Conference CA Section: 18 (Animal Nutrition) Section cross-reference(s): 1, 14 DES quinone [5664-37-9] was prepd. by DES (synthetic estrogen) [56-53-1] oxidn., and this compd. was then reduced by ascorbic acid [50-81-7], yielding b-dienestrol [35495-11-5] (rearrangement) and approx. equimolar amts. of cis-DES [22610-99-7] and trans-DES [56-53-1]. In male Syrian hamsters, renal tumors induced by DES (DES 90, cholesterol 10% pellets) s.c. implants were inhibited dose-dependently by dietary ascorbic acid (1 or 2.5 g/kg feed). .