Detail of "3616-42-0"

Reference

Immunoadjuvanticity of a lipid A preparation from Salmonella typhosa

Immunoadjuvanticity of a lipid A preparation from Salmonella typhosa. Liu, Xuebo; Xie, Jinguang (Inst. Microbiol. Epidemiol., Acad. Mil. Med. Sci., Beijing, Peop. Rep. China). Zhonghua Weishengwuxue He Mianyixue Zazhi, 5(2), 106-9 (Chinese) 1985. CODEN: ZWMZDP. ISSN: 0254-5101. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Free lipid A, prepd. by acid hydrolysis from S. typhosa lipopolysaccharide (LPS), was a heterogeneous glycolipid composed of fatty acids and glucosamine phosphate [3616-42-0]. Of the 8 compds. sepd. by HPLC, the simplest one was composed of 1 kind of fatty acid only, but the others were composed of 5-6 fatty acids. All of the free lipids A prepns. had good immunoadjuvant activities which were comparable with the parent LPS on a wt. basis. However the lethal toxicity of free lipid A in mice was lower than that of LPS.

The sugar phosphotransferase system of Streptomyces coelicolor is regulated by the GntR-family regulator DasR and links N-acetylglucosamine metabolism to the control of development

All Rights Reserved. The sugar phosphotransferase system of Streptomyces coelicolor is regulated by the GntR-family regulator DasR and links N-acetylglucosamine metabolism to the control of development. Rigali, Sebastien; Nothaft, Harald; Noens, Elke E. E.; Schlicht, Maximilian; Colson, Sevrine; Mueller, Marisa; Joris, Bernard; Koerten, Henk K.; Hopwood, David A.; Titgemeyer, Fritz; van Wezel, Giles P. ( Department of Biochemistry, Leiden Institute of Chemistry, Leiden University, Leiden 2300 RA, Neth.). Molecular Microbiology, 61(5), 1237-1251 (English) 2006 Blackwell Publishing Ltd. CODEN: MOMIEE. ISSN: 0950-382X. DOCUMENT TYPE: Journal CA Section: 10 (Microbial, Algal, and Fungal Biochemistry) Members of the soil-dwelling, sporulating prokaryotic genus Streptomyces are indispensable for the recycling of the most abundant polysaccharides on earth (cellulose and chitin), and produce a wide range of antibiotics and industrial enzymes. How do these organisms sense the nutritional state of the environment, and what controls the signal for the switch to antibiotic prodn. and morphol. development. Here, the authors show that high extracellular concns. of N-acetylglucosamine, the monomer of chitin, prevent Streptomyces coelicolor progressing beyond the vegetative state, and that this effect is absent in a mutant defective of N-acetylglucosamine transport. They provide evidence that the signal is transmitted through the GntR-family regulator DasR, which controls the N-acetylglucosamine regulon, including the pts genes ptsH, ptsI and crr needed for uptake of N-acetylglucosamine. 3616-42-0 and 56941-29-8 are also in the experiment. Deletion of dasR or the pts genes resulted in a bald phenotype. Binding of DasR to its target genes is abolished by glucosamine 6-phosphate, a central mol. in N-acetylglucosamine metab. Extracellular complementation expts. with many bld mutants showed that the dasR mutant is arrested at an early stage of the developmental program, and does not fit in the previously described bld signalling cascade. Thus, the authors are able to directly link carbon (and nitrogen) metab. to development, highlighting a novel type of metabolic regulator, which senses the nutritional state of the habitat, maintaining vegetative growth until changing circumstances trigger the switch to sporulation. This work, and the model it suggests, provide new leads towards understanding how microorganisms time developmental commitment. .