Detail of "3758-34-7"

Reference

Effect of steroid hormones on lipid synthesis in organs of the reproductive system of male rabbits

Effect of steroid hormones on lipid synthesis in organs of the reproductive system of male rabbits. Borisov, V. M. (USSR). Sb. Nauchn. Tr. - Grodn. S-kh. Inst., 27, 17-21 (Russian) 1976. CODEN: SNGSAZ. ISSN: 0432-6849. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) In rabbits, hydrocortisone (I) [50-23-7] (10 mg/kg/day for 15 days, i.m.) decreased wts. of testes, and prostate, Cowper's, and adrenal glands. Estradiol propionate (II propionate) [3758-34-7] (10 mg/kg/day for 15 days, i.m.) decreased testes, Cowper's gland, and adrenal wts., but not prostate wts. Testosterone propionate [57-85-2] (10 mg/kg/day for 15 days, i.m.) did not affect testes or prostate wts., but decreased Cowper's and adrenal gland wts. Chorionic gonadotropin [9002-61-3] (5 units/kg/day for 15 days, i.m.) did not affect testes wts., increased prostate and Cowper's gland wts., and decreased adrenal wts. I increased nonesterified cholesterol and fatty acids and decreased triglyceride levels in the testes. Testosterone decreased and gonadotropin increased the content of esterified cholesterol in the testes.

The interaction of C-17 esters of estradiol with the estrogen receptor

The interaction of C-17 esters of estradiol with the estrogen receptor. Janocko, Laura; Larner, Janice M.; Hochberg, Richard B. (Sch. Med., Yale Univ., New Haven, CT 06510, USA). Endocrinology (Baltimore), 114(4), 1180-6 (English) 1984. CODEN: ENDOAO. ISSN: 0013-7227. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Various C-17 alkyl esters of estradiol (E2) were tested as ligands for the estrogen receptor (rabbit uterine cytosol).Several reagents with their cas registry numbers 3758-34-7 and 82204-96-4 are used here. The naturally occurring fatty acid esters, represented by E2-17-stearate [82205-00-3], E2-17-palmitate [5776-45-4], and E2-17-arachidonate [82204-96-4] did not compete with [3H]E2 for receptor-binding sites. [3H]E2-17 stearate did not bind in a specific or saturable manner to uterine cytosol. On the other hand, the long-acting pharmacol. esters: E2-17-acetate [1743-60-8], E2-propionate [3758-34-7], E2-valerate [979-32-8] etc., were highly effective in competing for the binding of [3H]E2 to the uterine receptor. However, these short-chain esters were hydrolyzed to E2 when incubated with uterine cytosol under the conditions used for the binding assay. Consequently, [3H]E2-17-acetate and [3H]E2-17-valerate were tested as ligands in an assay in which the hydrolytic activity was minimized by partially purifying the receptor with (NH4)2SO4 pptn. and by limiting the duration of the incubation. In this assay there was no specific binding of the C-17-valerate ester. There was a relatively small amt. of specifically bound radioactivity in the incubation of the acetate ester, but on HPLC anal., the specifically bound steroid was found to be E2 and not the ester. Thus, the C-17 esters of E2, from C2 to the long-chain fatty acids, all of which are potent long-acting estrogens, exert their estrogenic effects only after hydrolysis to the free C18 steroid. .