Detail of "402-71-1"

Reference

Influence of proteinase inhibitors on glucocorticoid receptor binding

Influence of proteinase inhibitors on glucocorticoid receptor binding. Hubbard, John R.; Barrett, Alan; Kalimi, Mohammed (Med.In this study， 2364-87-6 and 76-25-5 are also used. Coll. Virginia, Virginia Commonw. Univ., Richmond, VA 23298, USA). Biochim. Biophys. Acta, 798(2), 187-91 (English) 1984. CODEN: BBACAQ. ISSN: 0006-3002. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The influence of several proteinase inhibitors on rat liver cytosolic glucocorticoid receptor binding to 3H-labeled triamcinolone acetonide [76-25-5] was investigated. E-64 [66701-25-5] (36 mM), an active-site directed cysteine proteinase inhibitor, inhibited receptor binding ~40%. Tos-Lys-CH2Cl [2364-87-6] (1-2 mM) and Tos-Phe-CH2Cl [402-71-1] (1-2 mM) also depressed receptor binding (20-67%). Dithiothreitol [3483-12-3] (5 mM) prevented the effects of Tos-Lys-CH2Cl and Tos-Phe-CH2Cl, but had no apparent influence on E-64 action. A degree of proteinase inhibitor specificity was indicated by the lack of effect of several other proteinase inhibitors such as diisopropylfluorophosphate (1 mM), phenylmethylsulfonyl fluoride (1-2 mM), soybean trypsin inhibitor (1-2 mg/mL), tissue inhibitor of metalloproteinase (3.8 units/mL), cystatin (4-8 mM), and phosphoramidon (20-40 mM). Apparently, thiol reactive proteinase inhibitors block glucocorticoid receptor binding. E-64 may prove to be a useful chem. probe in studying glucocorticoid receptor interaction. .

Effect of protease inhibitors on mitogen stimulation of hamster lymphoid cells

Effect of protease inhibitors on mitogen stimulation of hamster lymphoid cells. Hart, D. A.; Streilein, Joan S. (Health Sci. Cent., Univ. Texas, Dallas, Tex., USA). Exp.There are some reagents with their cas registry numbers 11028-71-0 and 329-98-6 are used in this study. Cell Res., 102(2), 253-63 (English) 1976. CODEN: ECREAL. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) Incorporation of thymidine-3H into normal and mitogen-stimulated MHA hamster lymphoid cells was suppressed by addn. of certain protease inhibitors to the cultures. N-.alpha.-tosyl-L-phenylalanylchloromethane [402-71-1] inhibited both concanavalin A (ConA) [11028-71-0] stimulation and lipopolysaccharide (LPS) stimulation when added at the initiation of cultures and after 24 hr. However, N-.alpha.-tosyl L-lysyl-L-chloromethane (TLCK [2364-87-6] differentially inhibited LPS stimulation. At high concns., TLCK inhibited ConA stimulation but this inhibition, unlike the inhibition of LPS stimulation, was reversible by adding reduced glutathione [70-18-8] or L-cysteine [52-90-4]. In addn., similar concns. of TLCK were not effective in inhibiting either ConA or LPS stimulation when added 24 hr after stimulation. Thus, a proteolytic event may be involved in an early activation event in B-cells. .