Detail of "41276-95-3"

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Ultrastructure of Kurloff body proteoglycans after high pressure freezing, cryosubstitution and postembedding staining with cuprolinic blue

Ultrastructure of Kurloff body proteoglycans after high pressure freezing, cryosubstitution and postembedding staining with cuprolinic blue. Landemore, Gerard; Quillec, Michele; Izard, Jacques (Lab.Several substances are used for example 41276-95-3 which is its cas registry number. Histol. Biol. Cell., Univ. Caen-Basse Normandie, Caen F14032, Fr.). Glycobiology, 6(8), 817-822 (English) 1996 Oxford University Press. CODEN: GLYCE3. ISSN: 0959-6658. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Very high pressure freezing and cryosubstitution of Kurloff cells preserves the ultrastructural morphol. of Kurloff bodies, particularly the myelin figures, as shown by embedding in epoxy resin and conventional postembedding staining. It also preserves the Kurloff body proteoglycans as more expanded spindle-like shapes than does fixation with formaldehyde at atm. pressure. But, proteoglycans were not discernible in the Kurloff body matrix on either unstained or conventionally stained thin sections. The Kurloff body skeleton of proteoglycans in their native expanded shape was stained with the electron-dense cationic ministain cuprolinic blue, using thin sections embedded in LR white. The mean equatorial diam. of the spindles was 20-30 nm, while the collapsed filaments produced by aldehyde fixation were about 10-15 nm wide. The spindles were often about 200-300 nm long but could be much longer, depending on the plane of the section. Thus, high pressure freezing, freeze substitution, embedding in LR white, and staining with cationic dyes such as phthalocyanins seems to be a convenient way of visualizing intracellular proteoglycans that are well preserved and in very much like their native expanded state. .

Cuprolinic Blue: a specific dye for single-stranded RNA in the presence of magnesium chloride

Cuprolinic Blue: a specific dye for single-stranded RNA in the presence of magnesium chloride. II. Practical applications for light microscopy. Mendelson, Dan; Tas, Johan; James, Jan (Jan Swammerdam Inst., Univ. Amsterdam, Amsterdam 1054 BW, Neth.). Histochem. J., 15(11), 1113-21 (English) 1983. CODEN: HISJAE. ISSN: 0018-2214. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Cuprolinic Blue was used for staining of nucleic acids in isolated rat hepatocytes and various rat tissue (liver, testis, cerebellum) tissues. Without added cations, Cuprolinic Blue stains both DNA and RNA, whereas in the presence of 1M MgCl2, Cuprolinic Blue specifically stains single-stranded RNA only. The total RNA can be stained after removal of DNA by DNAase digestion. Fixation in a modified Carnoy soln. gave optimal staining results in all cases tested. By cytophotometry at 635 nm, a reliable and reproducible relative est. can be obtained of the total nucleic acid content, the total RNA content, and the amt. of single-stranded RNA alone per cell.Except for chemicals metioned above, 41276-95-3 and 7786-30-3 are also used. .