Reference

Determination of glucocorticoids by liquid chromatography

Determination of glucocorticoids by liquid chromatography. III. Application to ointments and a cream containing cortisone acetate, dexamethasone acetate, fluorometholone, and betamethasone valerate. Tokunaga, Hiroshi; Kimura, Toshio; Kawamura, Jiro (Natl. Inst. Hyg. Sci., Tokyo 158, Japan). Chem. Pharm. Bull., 32(10), 4012-16 (English) 1984. CODEN: CPBTAL. ISSN: 0009-2363. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Section cross-reference(s): 2 To sep. cortisone acetate (I) [50-04-4], dexamethasone acetate [1177-87-3], fluorometholone [426-13-1], and betamethasone valerate [2152-44-5] in ointments and a cream from the base and excipients, a silica gel column pretreatment was carried out (pre-screening procedure) and then glucocorticoids were successfully detd. by HPLC on a LiChrosorb RP-18 column. By the use of the pre-screening procedure, the base, excipients, and other active ingredients (except glycyrrhetinic acid [471-53-4]) in com. prepns. were almost completely removed, and hence a lowering of the efficiency of the LiliChrosorb RP-18 column and blocking of the flow of the mobile phase in column caused by the base remaining on the top of the column could be avoided. The contents of glucocorticoids in various com. prepns. were 95.7-102.5% of the labeled amts.

Densitometric determination of dexamethasone, fluocinolone, fluorometholone and methylprednisolone

Densitometric determination of dexamethasone, fluocinolone, fluorometholone and methylprednisolone. Zivanovic, Ljiljana (Zavod Farm. Hem., Farm. Fak., Belgrade, Yugoslavia). Arh. Farm., 36(6), 307-12 (Serbo-Croatian) 1986. CODEN: ARFMAC. ISSN: 0004-1963. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Dexamethasone [50-02-2], fluocinolone acetonide [67-73-2], fluorometholone [426-13-1], and methylprednisolone hemisuccinate [2921-57-5] were detd. in pharmaceuticals by densitometry. Corticosteroids were sepd. by TLC by using CHCl3-Me2CO as the mobile phase. The spots obtained were developed with p-acetylaminobenzaldehyde thiosemicarbazone in acidic medium and the drugs were directly detd. at 560-580 nm. The method is simple, rapid (10-20 min) and precise with coeff. of variation of 0.010-0.202%.