Reference

Alkylphthalides isolated from Ligusticum wallichii Franch and their in vitro inhibitory effect on rat uterine contraction induced by prostaglandin F2a

Alkylphthalides isolated from Ligusticum wallichii Franch and their in vitro inhibitory effect on rat uterine contraction induced by prostaglandin F2a. Ko, Wun-Chang; Lin, Song-Chow; Yeh, Ching-Ying; Wang, Yao-Tung (Dep. Pharmacol., Taipei Med. Coll., Taipei, Taiwan). T'ai-wan I Hsueh Hui Tsa Chih, 76(9), 669-77 (English) 1977. CODEN: TIHHAH. ISSN: 0371-7682. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Section cross-reference(s): 62 Butylidenephthalide (I) [551-08-6], ligustilide (II) [4431-01-0], and butylphthalide (III) [6066-49-5] were isolated from the neutral oil fraction of L. wallichii following purifn., and the structures of these alkylphthalides were detd. by gas chromatog. and mass spectrometry. All 3 compds. inhibited PGF2a-induced contraction of rat (nonpregnant) uterus in vitro. Alkyl substitution at C-3 increased the inhibitory activity probably due to an increase in lipid soly. The D3-double bond was also an important structural feature for the inhibitory effect. I was more active than II, possibly due to the presence of arom. character in I. L. wallichii was more active than Cnidium officinale, possibly due to the higher content of I in L. wallichii.

Quantitative determination of ligustilide and butylidenephthalide in touki (Angelica Radix) and senkyu (Cnidii Rhizome) by high-performance liquid chromatography

Quantitative determination of ligustilide and butylidenephthalide in touki (Angelica Radix) and senkyu (Cnidii Rhizome) by high-performance liquid chromatography. Yamagishi, Takashi (Hokkaido Inst. Public Health, Sapporo 060, Japan). Hokkaidoritsu Eisei Kenkyushoho, (32), 12-16 (Japanese) 1982. CODEN: HOEKAN. ISSN: 0441-0793. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Ligustilide (I) [4431-01-0] and butylidenephthalide (II) [551-08-6] were detd. in several samples of Angelica root and Cnidium rhizomes by high-performance liq. chromatog. A powd. sample was extd. with hexane at 40-50° for 30 min. The hexane soln. was analyzed on a high-performance liq. chromatog. with Unisil CP 5 mm column, hexane-CH2Cl2 () solvent, 1.5 mL/min flow rate and detection at 240 nm. Amts. of I and II in the tested samples were 0.399 and 0.018 in Senkyu originating from Cnidium officinale Makino of Hokkaido, 0.814-0.909 and 0.045-0.046 in 2 samples of Senkyu from Ligusticum chaunxiong Hort of Formosa, 0.545 and 0.080 in Senkyu from L. chaunxiong of Hong Kong, 0.175 and 0.028 in Senkyu from Viet-Nam, 0.089-0.109 and 0.005-0.006 in Touki from Angelica acutiloba Kitagawa of Hokkaido marketed in 1978, 0.106 and 0.004 by Touki from A. acutiloba var sugiyamae Hikino of Hokkaido, 0.042-0.045 and 0.005-0.007 in 2 samples of Touki from A. acutiloba of Nara and Korea marketed in 1973, 0.627-0.700 and 0.036-0.098 by 2 samples of Touki from A. carmichaeli Debx. marketed in 1973-5 of China and Hong Kong, resp. The method allowed sep. detection of I, Z-II [72917-31-8], E-II [76681-73-7], butylphthalide [6066-49-5], cnidilide [3674-03-1], senkyunolide [63038-10-8], and neocnidilide [4567-33-3] by changing the detection wave length between 230 and 310 nm.