Reference

Natural sweetener separation

Natural sweetener separation. Matsushita, Susumu; Ikushige, Tetsuo (Toyo Soda Mfg. Co., Ltd., Japan). Japan. Kokai JP 53038669 8 Apr 1978 Showa, 5 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: A23L001-22. APPLICATION: JP 76-109856 16 Sep 1976. DOCUMENT TYPE: Patent CA Section: 17 (Foods) Sweeteners such as phyllodulcin [21499-23-0], stevioside [57817-89-7], and glycyrrhetinic acid [471-53-4] are individually or simultaneously fractionated by column chromatog. on a porous cross-linking polymer gel having a pore diam. of <2.5 ′ 102 ? by using nonaq. solvents; this method gives more rapid sepn. of natural sweeteners than conventional methods. Thus, 1 g each of stevioside, glycyrrhetinic acid, phyllodulcin, and Na saccharin [128-44-9] were dissolved in 1 L THF. The mixt. was fractionated on a porous styrene gel column by using THF as eluent. The 4 components were sepd. sharply in a short time.

High-performance liquid chromatographic determination of glycyrrhizin and glycyrrhetinic acid in biological materials

High-performance liquid chromatographic determination of glycyrrhizin and glycyrrhetinic acid in biological materials. Ichikawa, Tsutomu; Ishida, Shiro; Sakiya, Yoko; Akada, Yoshinobu (Fac. Pharm. Sci., Tokushima Bunri Univ., Tokushima 770, Japan). Chem. Pharm. Bull., 32(9), 3734-8 (English) 1984. CODEN: CPBTAL. ISSN: 0009-2363. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) HPLC methods were developed for the detn. of glycyrrhizin (G) [1405-86-3] and glycyrrhetinic acid (GA) [471-53-4] in blood, plasma bile, urine, and visceral tissues of rats. The detection limit for G and GA was 0.125 mg/mL or per g wet wt. The precision and sensitivity of the assay appear to be satisfactory for detn. of these compds. in biol. materials. Utilizing this method, the plasma disposition, tissue distribution, and biliary and urinary excretions of G and GA were studied after a bolus i.v. administration of G or GA to rats. Plasma disposition followed a 2-compartment model for both G and GA with b-elimination half-lives of approx. 50 and 80 min, resp. The cumulative amts. of G and GA excreted in bile during 24 h after an i.v. dose were 88.5 and 0.36% of the dose, resp., and those excreted in urine were, resp., only 4.8 and 0.03% of the dose. These compds. were distributed in visceral tissues at lower concns. than in blood.