Detail of "481-85-6"

Reference

Inactivation of the infectivity of

Inactivation of the infectivity of .Some chemicals with cas registry numbers like 84-80-0 and 481-85-6 are also used.vphi.X174 DNA with some quinone derivatives. Komano, Tohru; Morita, Junji (Dep. Agric. Chem., Kyoto Univ., Kyoto, Japan). Nucleic Acids Res., Spec. Publ., 2(Symp. Nucleic Acids Chem., 4th, 1976), 131-3 (English) 1976. CODEN: NARPD6. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Interactions) Section cross-reference(s): 6 Menadione (I) [58-27-5] inactivated the infectivity of .vphi.X174 DNA, however reduced I [481-85-6] more effectively inactivated .vphi.X174 DNA. Reduced phylloquinone [572-96-3] and ubiquinone-6 [1065-31-2] also inactivated .vphi.X174 DNA, but phylloquinone [84-80-0] did not affect .vphi.X174 DNA at <2 .times. 10-4M. Inactivation of .vphi.X174 DNA by reduced I was caused partially by DNA strand breakage. But DNA strand breakage by I was not obsd. .

Effects of Site-Directed Mutations on Heme Reduction in Escherichia coli Nitrate Reductase A by Menaquinol: A Stopped-Flow Study

Effects of Site-Directed Mutations on Heme Reduction in Escherichia coli Nitrate Reductase A by Menaquinol: A Stopped-Flow Study. Zhao, Zhongwei; Rothery, Richard A.; Weiner, Joel H. (CIHR Membrane Protein Research Group, Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Can.). Biochemistry, 42(48), 14225-14233 (English) 2003 American Chemical Society. CODEN: BICHAW. ISSN: 0006-2960. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) The authors have studied the effects of site-directed mutations in Escherichia coli nitrate reductase A (NarGHI) on heme redn. by a menaquinol analog (menadiol) using the stopped-flow method. For NarGHIH66Y and NarGHIH187Y, both lacking heme bL but having heme bH, the heme redn. by menadiol is abolished. For NarGHIH56R and NarGHIH205Y, both without heme bH but with heme bL, a smaller and slower heme redn. compared to that of the wild-type enzyme is obsd. These results indicate that electrons from menadiol oxidn. 71-00-1 and 481-85-6 which are cas registry numbers are also used here. are transferred initially to heme bL. A transient species, likely to be assocd. with a semiquinone radical anion, was generated not only on redn. of the wild-type enzyme as obsd. previously (1) but also on redn. of NarGHIH56R and NarGHIH205Y. The inhibitors 2-n-heptyl-4-hydroxyquinoline-N-oxide and stigmatellin both have significant effects on the redn. kinetics of NarGHIH56R and NarGHIH205Y. The authors have also investigated the reoxidn. of menadiol-reduced heme by nitrate in the mutants. Compared to the wild type, no significant heme reoxidn. is obsd. for NarGHIH56R and NarGHIH205Y. This result indicates that a single mutation removing heme bH blocks the electron-transfer pathway from the subunit NarI to the catalytic dimer NarGH. .