Reference

Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence

Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence. Klingler, W.; Haupt, O.; Von Postel, G.; Knuppen, R. (Inst. Biochem. Endokrinol., Med. Hochsch. Luebeck, Luebeck D-2400, Fed. Rep. Ger.). Steroids, 42(2), 123-36 (English) 1983. CODEN: STEDAM. ISSN: 0039-128X. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) A chemiluminescence immunoassay for detn. of estriol [50-27-1] in the blood serum of women is described. The method involves the detn. of bound estriol-luminol conjugate upon oxidn. with a H2O2/microperoxidase system. The prepn. of 6-oxoestriol-6-(O-carboxymethyl)oxime-aminobutylethylisoluminol [82546-64-3] is described. 7323-86-6 and 66612-29-1 are also in the experiment. The method is specific, accurate, and precise with inter- and intra-assay coeffs. of variation of 8.4-10.6 and 8.4-12.9, resp., and a sensitivity of 700 pM. On application of the method to detn. of serum estriol in pregnant women, results correlated well with those of conventional RIA. Estriol serum levels are given for women during normal pregnancy and in placenta insufficiency. .

The effect of phenobarbital on serum hormone levels and their diurnal variations in pregnancy

The effect of phenobarbital on serum hormone levels and their diurnal variations in pregnancy. Luoma, P. V.; Heikkinen, J. E.; Ylostalo, P. R. (Dep. Med. Obstet. Gynecol., Univ. Oulu, Oulu SF-90220/22, Finland). Pharmacology, 28(4), 211-15 (English) 1984. CODEN: PHMGBN. ISSN: 0031-7012. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) The effect of phenobarbital (PB) [50-06-6] on serum hormones in late pregnancy was investigated. Eighteen pregnant women were studied before and after 2 wk of PB treatment (100 mg orally, daily at 8 p. 9002-62-4 and 50-28-2 which are cas registry numbers are also used here.m.) by measuring serum estradiol [50-28-2], estriol [50-27-1], progesterone [57-83-0], testosterone [58-22-0], prolactin [9002-62-4] and cortisol [50-23-7] concns. Seven pregnant women without PB therapy served as controls. Estradiol levels in the morning decreased, whereas those of estriol increased both in the morning and evening during PB treatment. The estriol/estradiol ratio increased in women on PB. PB affected the diurnal variation of hormones; the decrease in estradiol concn. between 8 a.m. and 8 p.m. was eliminated and the decrease in estriol concn. became more clear. PB did not significantly affect the serum levels of the other hormones investigated. PB may affect the metab. of sex hormones. .