Reference

Enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by Pseudomonas sp

Enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by Pseudomonas sp. strain B13. Lehrbach, Philip R.; Zeyer, Josef; Reineke, Walter; Knackmuss, Hans Joachim; Timmis, Kenneth N. (Dep. Biochim. Med., Univ. Geneve, Geneva CH-1211/4, Switz.). J. Bacteriol., 158(3), 1025-32 (English) 1984. CODEN: JOBAAY. ISSN: 0021-9193. DOCUMENT TYPE: Journal CA Section: 3 (Biochemical Genetics) Section cross-reference(s): 10 DNA fragments contg. the xylD and xylL genes of the TOL plasmid pWW0-161 of P. putida that code for the catabolic enzymes toluate 1,2-dioxygenase [9059-18-1] and dihydrodihydroxybenzoic acid dehydrogenase [9059-28-3], resp., and the nahG gene of the NAH plasmid NAH7 that codes for salicylate hydroxylase [9059-28-3] were cloned onto plasmid pBR322. Deletion and insertion mutagenesis were used to localize these genes with respect to crucial endonuclease cleavage sites. The pBR322-based plasmids were ligated to the broad-host-range cloning vector pKT231 or derivs. of it, and the hybrid plasmids were introduced into Pseudomonas B13(WR1), a bacterium able to degrade 3-chlorobenzoate [16887-60-8] but not 4-chlorobenzoate [74-11-3], 3,5-dichlorobenzoate [51-36-5], salicylate [69-72-7], or chlorosalicylates. The clone xylD gene expanded the catabolic range of WR1 to include 4-chlorobenzoate, whereas the cloned xylD-xylL genes enabled the isolation of derivs. of WR1 that degraded 3-chlorobenzoate, 4-chlorobenzoate, and 3,5-dichlorobenzoate. The cloned nahG gene extended the catabolic range of WR1 to include salicylate and 3- [5106-98-9], 4- [321-14-2], and 5-chlorosalicylate [321-14-2].

Studies on the metabolism of benthiocarb

Studies on the metabolism of benthiocarb. Part VII. Metabolic fate of benthiocarb herbicide in plants. Nakamura, Yasuo; Ishikawa, Kanji; Kuwatsuka, Shozo (Fac. Agric., Nagoya Univ., Nagoya, Japan). Agric. Biol. Chem., 41(9), 1613-20 (English) 1977. CODEN: ABCHA6. DOCUMENT TYPE: Journal CA Section: 4 (Toxicology) The metabolic fate of 14C-labeledbenthiocarb (I) [28249-77-6] in rice and barnyard millet at the 4-leaf stage was studied. I was degraded rapidly after absorption through the root. Most of the radioactivity in the plants was extd. with aq. acetone. The radioactivity in the water-sol. fraction and the aq. acetone-unextractable residue increased with decreasing I. Desethyl benthiocarb [39918-94-0], S-4-chlorobenzyl thiocarbamate [64917-79-9], 4-chlorobenzoic acid [74-11-3], 2-hydroxybenthiocarb,desethyl 2-hydroxybenthiocarb [64917-80-2], 4-chloro-2-hydroxybenzyl alc. [64917-81-3], and 4-chlorosalicylic acid [5106-98-9], were identified as the metabolic products by thin-layer chromatog. Five of them having hydroxy group and (or) carboxyl group, and 4-chlorobenzyl alc. were also found as conjugation products. The same metabolic products were also found in both the root and foliage of both plants. There was little difference in the metabolic pattern of both plants.