Reference

In-vitro human skin penetration of the fragrance material geranyl nitrile

All Rights Reserved. Some chemicals with cas registry numbers like 5146-66-7 are also used. In-vitro human skin penetration of the fragrance material geranyl nitrile. Brain, K. R.; Green, D. M.; Lalko, J.; Api, A. M. (An-eX, Cardiff CF10 3XF, UK). Toxicology in Vitro, 21(1), 133-138 (English) 2007 Elsevier Ltd. CODEN: TIVIEQ. ISSN: 0887-2333. DOCUMENT TYPE: Journal CA Section: 62 (Essential Oils and Cosmetics) In-vitro human skin permeation and distribution of geranyl nitrile (GN) was detd. using epidermal membranes following application (5 ml/cm2) in 70% ethanol, under nonocclusive conditions, at max. in-use concn. (1%). Permeation was measured (12 time-points over 24 h) using 6% (w/v) Oleth-20 in pH 7.4 phosphate buffered saline as receptor. Permeation of ref. benzoic acid was assessed using the same skin donors. Overall recovery of GN at 24 h was low (14.1±0.4%) due to evapn. Evaporative loss of GN from polytetrafluoroethylene (PTFE) sheet, under the same conditions was rapid (93% over 24 h) although this overestimated loss during permeation where evapn. competed with uptake. At 24 h, 1.89±0.15 mg/cm2 GN, (3.74±0.30% of applied dose) (mean ± std. error, SE, n = 12), had permeated. Following rapid initial permeation, the absorption plateaued due to depletion. Levels of GN in the epidermis (plus any remaining stratum corneum after tape stripping), filter paper membrane support and receptor fluid were combined (as per SCCNFP guidelines) to produce a total absorbed dose value of 4.72±0.32%. Systemic exposure resulting from the use of GN as a fragrance ingredient, under nonocclusive conditions, would be low based on the currently reported use levels. .