Detail of "51481-10-8"

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Method for detecting production of zearalenone, zearalenol, T-2 toxin, and deoxynivalenol by Fusarium isolates

Method for detecting production of zearalenone, zearalenol, T-2 toxin, and deoxynivalenol by Fusarium isolates. Richardson, Kurt E.; Hagler, Winston M., Jr.; Hamilton, P. B. (Dep. Poultry Sci., North Carolina State Univ., Raleigh, NC 27695, USA).Chemicals with cas numbers 21259-20-1 and 36455-72-8 also play role. Appl. Environ. Microbiol., 47(4), 643-6 (English) 1984. CODEN: AEMIDF. ISSN: 0099-2240. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) Section cross-reference(s): 4, 10 Three methods for detecting toxigenic fusaria in culture were compared by using known producers of zearalenone [17924-92-4], a- [36455-72-8], and b-zearalenol [71030-11-0], T-2 toxin [21259-20-1], and deoxynivalenol [51481-10-8]. Moist, autoclaved rice cultures of known toxigenic isolates grown in 20-mL tubes yielded oily exts. contg. compds. which interfered with qual. and quant. anal. for the mycotoxins. Vermiculite moistened with nutrient broth in 20-mL tubes yielded a much cleaner ext. Growing the fungi on a liq. medium required a shorter incubation period, but yields of T-2 toxin and deoxynivalenol were low and variable, and the method required greater space in the incubator. Screening of the exts. by TLC with colorimetric spray reagents to detect the presence of these toxins permitted redn. in the no. of exts. quantified by the more lengthy gas chromatog. method. Culturing in nutrient broth on vermiculite in tubes coupled to a qual. screen before quantitation proved to be a convenient, inexpensive, and relatively rapid method that enabled reliable screening of a large no. of Fusarium isolates for toxin prodn. as compared with prior methods. .

Production of deoxynivalenol and related compounds in liquid culture by Fusarium graminearum

Production of deoxynivalenol and related compounds in liquid culture by Fusarium graminearum. Miller, J. D.; Taylor, A.; Greenhalgh, R. (Chem. Biol. Res. Inst., Agric. Canada, Ottawa, ON K1A 0C6, Can.). Can. J. Microbiol., 29(9), 1171-8 (English) 1983. CODEN: CJMIAZ. ISSN: 0008-4166. DOCUMENT TYPE: Journal CA Section: 16 (Fermentation and Bioindustrial Chemistry) A liq. culture method for the prodn. of deoxynivalenol [51481-10-8] and related compds. by F. graminearum was developed. Major factors which stimulate the biosynthesis of these compds. include reduced O2 levels, depletion of carbohydrate in the medium, pH, and possibly a low concn. of an org. N source. Isolates of F. graminearum were tested for the yields of 4 trichothecene mycotoxins and zearalenone [17924-92-4] in this system. The time course of 3-acetyldeoxynivalenol [50722-38-8], deoxynivalenol, and zearalenone in the fermn. was measured over a 21-day period against pH, glucose concn., protein, fungal biomass, and ergosterol. A new ester of deoxynivalenol, 15-acetyldeoxynivalenol [88337-96-6], is reported from North American isolates of F.Except for chemicals metioned above, 88337-96-6 and 50722-38-8 are also used. graminearum. .