Detail of "51548-48-2"

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Quantitative determation by the enzymic method

Quantitative determation by the enzymic method. (Toyo Jozo Co., Ltd., Japan). Jpn. Kokai Tokkyo Koho JP 59063198 A2 10 Apr 1984 Showa, 54 pp. (Japanese). (Japan). CODEN: JKXXAF. CLASS: IC: C12Q001-26; C12Q001-34; G01N033-50. APPLICATION: JP 82-173569 1 Oct 1982. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 4, 7 An enzymic-colorimetric method for the quant. detn. of I (where R1 = OH or NH2; R2, R3, R4, R5, R6 = H, halogen, lower alkyl, lower alkoxy, NH2, substituted amino, OH, carboxy; sulfo; R5 and R6 may form a linkage) is described. I is reacted with its complex in the presence of an oxidase (ascorbic acid oxidase [9029-44-1], laccase [80498-15-3], tyrosinase [9002-10-2]) and the reaction mixt. is analyzed by colorimetry for the detn. of I. Complexes such as 1-naphthol-2-sulfonic acid [567-18-0], phenol [108-95-2], 2,5-dimethylphenol [95-87-4], o-methylaniline [95-53-4], m-methylaniline [108-44-1], 4-chloro-1-naphthol [604-44-4], cleve's acid [51548-48-2], 1-hydroxy-2-naphthoic acid [86-48-6], o-chlorophenol [95-57-8], m-aminophenol [591-27-5], m-phenylenediamine [108-45-2], 2,6-dibromophenol [608-33-3], 2,6-dimethoxyphenol [91-10-1], 3,5-dimethylphenol [108-68-9], 2,6-dimethylphenol [576-26-1], 2,4-dimethylphenol [105-67-9], 2,3-dimethylphenol [526-75-0], 2,5-dimethylphenol [95-87-4], anthranilic acid [118-92-3], N,N-dimethylaniline [121-69-7], aniline [62-53-3], m-chloroaniline [108-42-9], o-aminophenol [95-55-6], 2-naphthol [135-19-3], m-bromoaniline [591-19-5], N,N-ethylhydroxyethylaniline [92-50-2], m-chlorophenol [108-43-0], p-chlorophenol [106-48-9] and p-bromophenol [106-41-2] can be used.There are some reagents like 9029-44-1 is used in this study. Thus, a sample contg. 2,6-dibromo-4-aminophenol [609-21-2] was treated with K 1-naphthol-2-sulfonate [832-49-5] in the presence of ascorbic acid oxidase at 37° for 10 min and the reaction mixt. was analyzed at 630 nm for the detn. of 2,6-dibromo-4-aminophenol. Based on these methods, activities of a-glucosidase [9001-42-7], cystine aminopeptidase [9031-41-8], a-chymotrypsin [9004-07-3], trypsin [9002-07-7], a-amylase [9000-90-2], alk. phosphatase [9001-78-9], esterase [9013-79-0] and endotoxin was detd. .