Reference

Specific bacterial inclusions in bone marrow cells indicate systematic lupus erythematosus, and treatment for lupus

All Rights Reserved. Specific bacterial inclusions in bone marrow cells indicate systematic lupus erythematosus, and treatment for lupus. Kallick, Charles A. (Sphingomonas Research Partners, L.P., USA). U.S. Pat. Appl. Publ. US 2007031820 A1 8 Feb 2007, 13pp. (English). (United States of America). CODEN: USXXCO. INCL: 435004000; 435034000. APPLICATION: US 2006-461900 2 Aug 2006. PRIORITY: US 2005-706125P 5 Aug 2005. DOCUMENT TYPE: Patent CA Section: 9 (Biochemical Methods) Section cross-reference(s): 1, 10, 14 A method for diagnosing for the presence of systemic lupus erythematosus (SLE) in a patient is disclosed. In accordance with this method, megakaryocytes present in bone marrow of a person suspected of having SLE are assayed for the presence of internal bacterial structures that specifically stain with an intercalating dye. The presence of those specifically stainable structures within the patient's megakaryocytes indicates that the patient has SLE. Treatment of an SLE patient with an antibiotic is contemplated. Treatment of a patient that has SLE comprises administering to that patient (i) an antibacterial amt. of a rifamycin along with an antibacterial amt. of a macrolide, (ii) an antibacterial amt. of a tetracycline, or (iii) an antibacterial amt. of a quinolone, or a mixt. of two or more of i, ii and iii. 389-08-2 and 51811-82-6 are just another two chemicals used in this study. The treatment is continued until the patient's megakaryocytes no longer contain specifically stainable structures, and until no further evidence of infection is present. .

Detection of DNA in gels by Giemsa's staining or acridine orange staining

Detection of DNA in gels by Giemsa's staining or acridine orange staining.Some chemicals with cas registry numbers like 51811-82-6 and 65-61-2 are also used. He, Mingwei; Wang, Guangping; Li, Jing (Central Lab., Xiangya Hospital, Hunan Medical Univ., Changsha, Peop. Rep. China). Hunan Yike Daxue Xuebao, 21(5), 463-464 (Chinese) 1996 Hunan Yike Daxue. CODEN: HYXBET. ISSN: 1000-5625. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 3 Giemsa's stain staining rendered DNA red and RNA blue, and acridine orange stained DNA and RNA displayed light yellow green fluorescence and the sensitivity was less than ethidium bromide and higher than Giemsa's stain. The results suggest that acridine orange stained DNA can be used in PCR. .