Reference

Pulse polarographic determination of anthraquinones in Polygonum cillinerve (Nakai) Ohwi

Pulse polarographic determination of anthraquinones in Polygonum cillinerve (Nakai) Ohwi. Zhang, Xiuqin; Xu, Lixin (Inst. Mater. Med., Chin. Acad. Med. Sci., Beijing, Peop. Rep. China). Yaoxue Xuebao, 19(7), 519-23 (Chinese) 1984. CODEN: YHHPAL. ISSN: 0513-4870. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Optimal conditions for the detn. of emodin (I) [518-82-1] and physcion [521-61-9] in the bulb of P. cillinerve by pulse polarog. were established. A 0.5-g sample was extd. with MeOH and the ext. was chromatographed on a silica gel G plate by using hexane-ether-EtOAc-CH2Cl2-formic acid (8:1.5:1:0.8:0.1) as eluent. Spots corresponding to free I and physion were scrapped, extd. with EtOH and an aliquot (1.0-4.0 mL) with addn. of EtOH, 4.0 mL LiCl (0.1M) and 1 drop of dil. NH4OH was analyzed by pulse polarog. The current was linearly correlated to the concn. in the range of 6.4 ′ 10-6-3.1 ′ 10-5 and the relative std. deviation was >2%. For the detn. of conjugates, samples were hydrolyzed with HCl to give total I and total physcion. Based on these results (e.g. total I anthraquinones-free anthraquinones), conjugated anthraquinones were calcd. Thus, the bulk of P. cillinerve contained 0.05% free I, 3.10% free physcion, 0.4% I conjugate and 9.80% physcion conjugate.

Anthraquinones in Cassia siamea

Anthraquinones in Cassia siamea. Rai, P. P. (Dep. Pharm. Pharmacol., Ahmadu Bello Univ., Zaria, Nigeria). Curr. Sci., 46(23), 814-15 (English) 1977. CODEN: CUSCAM. DOCUMENT TYPE: Journal CA Section: 63 (Pharmaceuticals) Anthraquinones of leaf and bark of C. siamea, identified by thin-layer chromatog., included chrysophanol (I) [481-74-3], physcion [521-61-9] (only in bank), rhein [478-43-3], chrysophanol anthrone [491-58-7], and chrysophanol dianthrone [17062-54-3] (last 2 in the bark). All the compds. were isolated by preparative thin-layer chromatog. and were further confirmed by their phys. data. Free and combined anthraquinones, detd. by colorimetry and densitometry, were 0.07-0.10% and 0.6-1.0%, resp.