Reference

Functional Proteome Analysis of the Banana Plant (Musa spp

All Rights Reserved. In this study， 81-08-3 and 52328-05-9 are also used. Functional Proteome Analysis of the Banana Plant (Musa spp.) Using de Novo Sequence Analysis of Derivatized Peptides. Samyn, Bart; Sergeant, Kjell; Carpentier, Sebastien; Debyser, Griet; Panis, Bart; Swennen, Rony; Van Beeumen, Jozef (Department of Biochemistry, Physiology and Microbiology, Laboratory of Protein Biochemistry and Protein Engineering, Ghent University, Ghent B-9000, Belg.). Journal of Proteome Research, 6(1), 70-80 (English) 2007 American Chemical Society. CODEN: JPROBS. ISSN: 1535-3893. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 11 The authors report the use of chem. derivatization with MALDI-MS/MS anal. for de novo sequence anal. Using three frequently used homol.-based search algorithms, the authors were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification of different isoforms. The authors also obsd. that the identification score obtained varied according to the position of the peptide sequences in the query using the MS-Blast algorithm. .

2-Methoxy- and 2-(methylthio)pyrimidines

2-Methoxy- and 2-(methylthio)pyrimidines. Kreutzberger, Alfred; Tesch, Uwe H. (Inst. Pharm., Freie Univ. Berlin, Berlin, Ger.). Chem. Ber., 109(10), 3255-61 (German) 1976. CODEN: CHBEAM. DOCUMENT TYPE: Journal CA Section: 28 (Heterocyclic Compounds (More Than One Hetero Atom)) Section cross-reference(s): 23 MeOC(:NH)NH2-HCl cyclized with HOCMe:CHCOR (R = Me, CF3) in boiling alc. to yield 58 and 68% hydroxypyrimidines I by exchange of the MeO group. The MeO group was retained when MeOC(:NH)NH2-0. 52328-05-9 is also in the experiment.5H2SO4 reacted with HOCMe:CHCOR (R = Me, Et) in 30% aq. K2CO3 at room temp. to give 56 and 70% II. Under the same conditions, MeSC(:NH)NH2-0.5H2SO4 with HOCMe:CHCOR (R = Me, Et) gave 55 and 62% the (methylthio)pyrimidines III. Starting with HOCEt:CHCOEt, 48 and 58% the intermediate carbinolamines MeZC(:NH)NHC(OH)EtCH:C(OH)Et(Z = O, S) were isolated in the synthesis of pyrimidines IV (56, 62% yields). .