Detail of "52399-83-4"

Reference

N-Iodoacetyltyramine: preparation and use in iodine-125 labeling by alkylation of sulfhydryl groups

Lin, Chaomei; Mihal, Kevin A. 52399-83-4 is just another one chemical used in this study.; Krueger, Rick J. (Dep. Biochem., Univ. Nebraska, Lincoln, NE 68583-0718, USA). Anal. Biochem., 179(2), 389-95 (English) 1989. CODEN: ANBCA2. ISSN: 0003-2697. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Prepn. and use of N-iodoacetyltyramine in generation of 125I-labeled compds. is described. The kinetics of alkylation of N-acetyllcysteine by N-iodoacetyltyramine (k2 = 3.0 M-1 s-1) and N-chloroacetyltyramine (k2 = 0.12 M-1 s-1) indicate that N-iodoacetyltyramine is more useful for labeling sulfhydryl-contg. compds. to high specific activity with 125I. Conditions for prepn. of carrier-free 125I-labeled N-iodoacetyl-3-monoiodotyramine in 50% yield based on starting iodide are described. The high degree of group specificity of N-iodoacetyl-3-monoiodotyramine reaction with sulfhydryl groups is demonstrated by the high reactivity toward sulfhydryl-contg. bovine serum albumin and low reactivity toward N-ethylmaleimide-blocked bovine serum albumin and IgG. 125I-labeled N-iodoacetyl-3-monoiodotyramine was also used to prep. an 125I-labeled ACTH deriv. that retains full biol. activity, further demonostrating the selectivity toward reactions with sulfhydryl groups. .