Reference

New reagent for vitamin B6 derivative formation in gas chromatography

New reagent for vitamin B6 derivative formation in gas chromatography. Patzer, Emmons M.; Hilker, Doris M. (Dep. Food Nutr. Sci., Univ. Hawaii-Manoa, Honolulu, Hawaii, USA). J. Chromatogr., 135(2), 489-92 (English) 1977. CODEN: JOCRAM. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Gas-chromatog. sepn. of 4 vitamin B6 derivs. consisted of converting them into hemiacetals with EtOH, refluxing at 125.degree. for 15 min, evapg. the excess EtOH at 70.degree. under N, and adding the new reagent N-methylbistrifluoroacetamide [685-27-8], followed by refluxing at 125.degree. for 20 min and injecting the samples onto a colun packed with 5% silicone oil on Chromosorb P and using flame ionization detection. The compds. derivatized were pyridoxine-HCl (I) [58-56-0], pyridoxamine-di-HCl [524-36-7], deoxypyridoxine-HCl [148-51-6] and pyridoxal-HCl [65-22-5]. The min. detectable amt. is .apprx.250 ng. The procedure is rapid, clean, and simple.

Neurotoxic effects of pyridoxine and analogs in rats

Neurotoxic effects of pyridoxine and analogs in rats. Frater-Schroeder, M.; Alder, S.; Zbinden, G. (Inst. Toxicol. 62511-99-3 and 59050-80-5 are also occured in this study., Fed. Inst. Technol., Zurich, Switz.). Proc. Eur. Soc. Toxicol., 17(Predict. Chronic Toxic. Short Term Stud., Proc. Meet., 1975), 277-84 (English) 1976. CODEN: PESTD5. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacodynamics) Section cross-reference(s): 18 Pyridoxine-HCl (I-HCl) [58-56-0], 3 active vitamin B6 forms (pyridoxamine-2HCl [524-36-7], pyridoxal-HCl [65-22-5], and pyridoxol phosphate-HCl [62511-96-0]) and 7 synthetic I analogs were injected repeatedly into female rats. Only I and pyridoxol phosphate caused neuromuscular deficit as demonstrated with the rotating rod method, and histopathol. lesions of the sciatic nerve. From these studies the structural requirements and the lipid-water distribution coeff. necessary for the neurotoxic action of I analogs were established. Based on in vitro model reactions a possible biomechanism for the neurotoxic effect of I is proposed. .