Reference

Semi-preparative high-performance liquid chromatographic isolation of soybean isoflavones

Semi-preparative high-performance liquid chromatographic isolation of soybean isoflavones. Farmakalidis, E.; Murphy, P. A. (Dep. Food Technol., Iowa State Univ., Ames, IA 50011, USA). J. Chromatogr., 295(2), 510-14 (English) 1984. CODEN: JOCRAM. ISSN: 0021-9673. DOCUMENT TYPE: Journal CA Section: 17 (Food and Feed Chemistry) The isoflavone-contg. Me2CO-1M HCl ext. of defatted soybean flakes, after cleanup with CHCl3-MeOH to remove phospholipids, was fractionated by semipreparative high-performance liq. chromatog. on a 250 ′ 9.4 mm Partisil ODS-3 C18 semi-preparative column with a 2 cm LiChrosorb RP-18 guard column and UV detection at 254 nm. After recycling twice through the column, daidzin [552-66-9] was crystd. from 50% MeOH and genistin [529-59-9] was pptd. out of a supersatd. soln. of 100% EtOH. The method was limited in that only 10 mg of the mixt. could be applied to the column and the max. soly. in MeOH was only 5 mg/mL with 30% sample recovery. However, the method was superior to conventional chromatog. methods in that it required fewer purifn. steps.

Effect of 2,4-D on the qualitative composition of yellow lupine flavonoids

Effect of 2,4-D on the qualitative composition of yellow lupine flavonoids. Laman, N. A. (Inst. Eksp. Bot. im. Kuprevicha, Minsk, USSR). Mekh. Deistviya Gerbits. Sint. Regul. Rosta Rast. Ikh Sud'ba Biosfere, Mater. Mezhdunar. Simp. Stran-Chlenov SEV, 10th, Volume 1, 117-20. Edited by: Sokolov, M. S. Akad. Nauk SSSR, Nauchn. Tsentr Biol. Issled.: Pushchino, USSR. (Russian) 1975. CODEN: 37NSA7. DOCUMENT TYPE: Conference CA Section: 5 (Agrochemicals) Spraying yellow lupine in the greenhouse with 2 kg 2,4-D (I) [94-75-7]/ha induced formation of methylenedioxyorobol-7-glucoside [52663-80-6] and genistein-7-glucoside [529-59-9], and of small amts. of genistein (II) [446-72-0] and methylenedioxyorobol (III) [40624-03-1] in the leaves and tips within 2 days after the spray. Seven days after the spray orobol-7-glucoside (IV-glucoside) [20486-33-3] appeared. The flavonoids induced by I contribute to the phytotoxic effect of I by oxidn. of orthodioxy groups and formation of orthoquinones which are more phytotoxic than their phenol analogs.