Reference

Simultaneous production of monoclonal antibodies to dehydroepiandrosterone, estradiol, progesterone and testosterone

Simultaneous production of monoclonal antibodies to dehydroepiandrosterone, estradiol, progesterone and testosterone.Some chemicals with cas registry numbers like 50-28-2 and 53-43-0 are also used. Fantl, V. E.; Wang, D. Y. (Imp. Cancer Res. Fund, London WC2A 3PX, UK). J. Endocrinol., 100(3), 367-76 (English) 1984. CODEN: JOENAK. ISSN: 0022-0795. DOCUMENT TYPE: Journal CA Section: 15 (Immunochemistry) Mice were immunized with a mixt. of 4 steroid antigens: dehydroepiandrosterone (DHA), estradiol, progesterone, and testosterone linked to bovine serum albumin through the 7, 6, 11a and 17b positions, resp. The response to immunization varied widely with no one mouse producing an optimal response to all 4 steroids. In the 2 fusion expts. performed, antibodies to all 4 antigens were developed. The immune response of the spleen donor was related to the relative nos. and quality of the antibodies produced to each steroid. Despite the structural identity of the progesterone and testosterone haptens, antibodies elicited in response to their resp. antigens could readily be distinguished from each other. From the large no. of monoclonal antibodies obtained, those most useful for RIA were 3 high affinity antibodies to estradiol and 2 antibodies raised against DHA but with high affinity for DHA sulfate. .

Study of estrogen synthesis by a chick embryo ovary using various radioactive precursors

Study of estrogen synthesis by a chick embryo ovary using various radioactive precursors. Weniger, J. P.; Chouraqui, J.; Zeis, A. (Lab. Zool. Embryol. Exp., Univ. Louis-Pasteur, Strasbourg 67000, Fr.). Reprod., Nutr.In this article, certain chemicals are used. Some of their cas registry numbers are 53-43-0 and 53-16-7 , Dev., 23(6), 995-1002 (French) 1983. CODEN: RNDED4. ISSN: 0181-1916. DOCUMENT TYPE: Journal CA Section: 12 (Nonmammalian Biochemistry) The ability of the 17-day-old chick embryo ovary to convert radioactive progresterone, testosterone, androstenedione, and dehydroepiandrosterone to estrone and estradiol was investigated. The conversion rate was highest with dehydroepiandrosterone (13%). Testosterone was the next best precursor (6.5%), closely followed by androstenedione (5.1%), with progesterone giving the lowest rate (0.9%). The synthesis of estrogens from dehydroepiandrosterone thus seemed to bypass testosterone and androstenedione. The level of estradiol synthesis was consistently higher than that of estrone. Testosterone was not formed from dehydroepiandrosterone. .