Detail of "53005-05-3"

Reference

DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid)

DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid). Hamasaki, Naotaka (Med. Sch., Fukuoka Univ., Fukuoka, Japan). Seitai no Kagaku, 35(6), 482-4 (Japanese) 1984. CODEN: SEKAA6. ISSN: 0370-9531. DOCUMENT TYPE: Journal; General Review CA Section: 4 (Toxicology) A review with 7 refs. on the mechanism of action of DIDS (I) [53005-05-3] (a compd. which selectively inhibited anion transport by erythrocyte membrane).

Effects of standard diuretics and ortho-vanadate on sodium transport across isolated frog skin

Effects of standard diuretics and ortho-vanadate on sodium transport across isolated frog skin. Eriksson, Oerjan (Dep. Drugs, Natl. Board Health Welfare, Uppsala, Swed.). Acta Physiol. Scand., 122(3), 249-60 (English) 1984. CODEN: APSCAX. ISSN: 0001-6772. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Frog (Rana temporaria) skins were studied in a Ussing type lucite chamber adapted to diminish tissue edge damage. The transepithelial elec. p.d., short-circuit current and d.c. (DC) resistance of skins mounted in this chamber were 56, 20 and 24% higher, resp., than those of skins mounted in a conventional chamber. Amiloride [2609-46-3], triamterene [396-01-0], ouabain [630-60-4] and Na3VO4 inhibited short-circuit current and net mucosal/serosal flux of 22Na. Amiloride and triamterene had rapid onsets of action and were effective only when administered to the mucosal (pond) side of the skin. Ouabain and Na3VO4 had slower onsets of action and were effective only when administered to the serosal side of the skin. Steady-state effects of these drugs were not reached within the 3-h period of the expts. The inhibitory effect of Na3VO4 was blocked by adding a disulfonic stilbene deriv. (DIDS) [53005-05-3] to the serosal side of the skin. Serosal prostaglandin E2 [363-24-6] stimulated the short-circuit current and decreased the DC resistance. Thiazides, acetazolamide [59-66-5] and loop diuretics had no effects on Na+ transport by frog skin. Thus, frog skin seems to be a useful model only in studies of the mode of action and the structure-activity relationship of diuretics which act by inhibiting Na entry or Na+-K+-ATPase activity.